| PCR-based diagnosis of surra-targeting VSG gene: experimental studies in small laboratory rodents and buffalo. | |
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MedLine Citation:
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PMID: 20388585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trypanosoma evansi, the causative organism of 'surra' expresses its variable surface glycoprotein (VSG) at early, middle and late stages of infection in animals. The variable antigenic nature of VSG caused by switching its expression type favours evasion from the host immune response and leads to chronic and persistent infection. Developing a polymerase chain reaction (PCR)-based diagnostic tool targeting the VSG gene is expected to be highly specific and sensitive for diagnosis of surra. Hence, in the present study, we have designed EXP3F/4R primer pair and amplified the 1.4 kb of VSG gene of T. evansi and studied the phylogenetic relationship by in silico analysis. The PCR method was standardised using another set of primer, DITRYF/R, and 400 bp was amplified from blood and tissue samples of experimentally infected animals. Applying the PCR method, we were able to detect as low as 0.15 trypanosomeml(-1). Considering the number of parasite-to-DNA concentration, the PCR method has a sensitivity of 0.015 pg ml(-1). The PCR could detect the presence of the parasite as early as 24h post-infection (p.i.) and 72 h p.i., respectively, in experimentally infected rats and buffalo. No amplification was observed with DNA of Babesia bigemina and Theileria annulata, indicating the primers are specific for T. evansi. The PCR method could detect the dog, lion and leopard isolates of T. evansi. Similarly, amplifying the DNA from the experimentally infected tissues was also found to be sensitive. Thus, the findings of this study favour the application of PCR over the parasitological methods for the detection of the early and/or chronic stage of surra in domestic and wild animals. |
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Authors:
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P P Sengupta; M Balumahendiran; V V S Suryanaryana; A G Raghavendra; B R Shome; M R Gajendragad; K Prabhudas |
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Publication Detail:
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Type: Journal Article Date: 2010-03-18 |
Journal Detail:
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Title: Veterinary parasitology Volume: 171 ISSN: 1873-2550 ISO Abbreviation: Vet. Parasitol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7602745 Medline TA: Vet Parasitol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 22-31 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore 560024, Karnataka, India. pinakiprasad_s@rediffmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Buffaloes / parasitology* Carrier State / diagnosis, parasitology* DNA, Protozoan / chemistry, genetics Male Membrane Glycoproteins / chemistry, genetics Mice Molecular Sequence Data Phylogeny* Polymerase Chain Reaction / veterinary Rats Rats, Wistar Sequence Alignment Sequence Analysis, DNA Trypanosoma / genetics, isolation & purification* Trypanosomiasis / diagnosis, parasitology, veterinary* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan; 0/Membrane Glycoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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