Document Detail


PCR-based assays of mendelian polymorphisms from anonymous single-copy nuclear DNA: techniques and applications for population genetics.
MedLine Citation:
PMID:  8098128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper outlines a PCR-based approach for population genetics that offers several advantages over conventional Southern blotting methods for revealing restriction-fragment-length polymorphisms (RFLPs) in nuclear DNA. Primers are constructed from clones isolated from a nuclear DNA library, and these primers subsequently are employed in in vitro syntheses of homologous regions. Amplified products are then screened directly for RFLPs by using gel-staining procedures. Population applications for this PCR-based approach, including potential strengths and weaknesses, are exemplified by two RFLP data sets generated to estimate (a) male-mediated gene flow in the green turtle (Chelonia mydas) and (b) geographic population genetic structure in the American oyster (Crassostrea virginica). Restriction assays of amplified products from 14 or 15 independent primer pairs in each species revealed polymorphisms at several loci that proved highly informative in the population genetic analyses. In general, the Mendelian polymorphisms produced by this PCR-based approach will provide useful genetic markers for population studies, particularly in situations where simpler and less expensive allozyme methods have failed, for whatever reason, to provide adequate information.
Authors:
S A Karl; J C Avise
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular biology and evolution     Volume:  10     ISSN:  0737-4038     ISO Abbreviation:  Mol. Biol. Evol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-06-09     Completed Date:  1993-06-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8501455     Medline TA:  Mol Biol Evol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  342-61     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Georgia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Nucleus / chemistry
DNA / genetics*,  isolation & purification
Enzymes / genetics
Gene Library
Genetic Markers
Genetics, Population*
Genotype
Male
Molecular Sequence Data
Ostreidae / genetics
Polymerase Chain Reaction*
Polymorphism, Restriction Fragment Length*
Turtles / genetics
Chemical
Reg. No./Substance:
0/Enzymes; 0/Genetic Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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