Document Detail


PCR assay for species-specific identification of Bacteroides thetaiotaomicron.
MedLine Citation:
PMID:  10747167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteroides thetaiotaomicron is the second most frequently encountered species of the anaerobes isolated from clinical specimens. We developed a PCR-based assay for the rapid identification of B. thetaiotaomicron. Specific primers were based on shared amplicons of about 1.2 kb generated from B. thetaiotaomicron by randomly amplified polymorphic DNA. This 1.2-kb fragment was sequenced and then used to design a set of PCR amplification primers. This PCR generated an amplification product of 721 bp, which was unique to all 65 isolates of B. thetaiotaomicron tested. There was no amplification with isolates of other bacterial species. Restriction enzyme digestion of the amplification product and dot blot hybridization further verified the specificity of the assay. These results suggest that this PCR assay targets a nucleotide sequence that is strongly conserved in B. thetaiotaomicron. This simple and rapid PCR assay provides a rapid and accurate method for identification of B. thetaiotaomicron and shows promise for the detection of B. thetaiotaomicron in clinical samples.
Authors:
L J Teng; P R Hsueh; J C Tsai; F L Chiang; C Y Chen; S W Ho; K T Luh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  38     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-17     Completed Date:  2000-05-17     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1672-5     Citation Subset:  IM    
Affiliation:
School of Medical Technology, National Taiwan University College of Medicine, Taipei, Taiwan. ltjeng@ha.mc.ntu.edu.tw
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF182955
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MeSH Terms
Descriptor/Qualifier:
Bacteroides / classification*,  genetics*
Bacteroides Infections / microbiology*
DNA Primers
Humans
Immunoblotting
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Species Specificity
Chemical
Reg. No./Substance:
0/DNA Primers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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