Document Detail


PCR amplification of triosephosphate isomerase gene of Giardia lamblia in formalin-fixed feces.
MedLine Citation:
PMID:  18693546     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Giardia lamblia (syn G. intestinalis, G. duodenalis) is the intestinal protozoan producing non-bacterial diarrhea most prevalent in the world. PCR genotype classification of Giardia in feces depends on the quality and quantity of purified DNA and the removal of a great number of inhibitors. The aim of this study was to adapt a PCR protocol to the amplification of the triosephosphate isomerase (tpi) gene of Giardia lamblia in formalin-fixed feces. The tpi gene of G. lamblia was amplified in 28 of the 34 analyzed samples (82.35%) and the B genotype was obtained in all cases. Two major modifications were implemented to improve the performance of PCR from formolated fecal matter. One of these improvements was the use of polyvinylpyrrolidone (PVP) and the other was the addition of bovine serum albumin (BSA). The PCR protocol used in this study showed an amplification percentage exceeding the values reported by other authors with high sensibility and specificity.
Authors:
Nora Molina; Daniela Polverino; Marta Minvielle; Juan Basualdo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Revista latinoamericana de microbiología     Volume:  49     ISSN:  0187-4640     ISO Abbreviation:  Rev. Latinoam. Microbiol.     Publication Date:    2007 Jan-Jun
Date Detail:
Created Date:  2008-08-12     Completed Date:  2008-10-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0242625     Medline TA:  Rev Latinoam Microbiol     Country:  Mexico    
Other Details:
Languages:  eng     Pagination:  6-11     Citation Subset:  IM    
Affiliation:
Cátedra de Microbiología y Parasitología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Animals
Feces / parasitology*
Giardia lamblia / genetics*
Humans
Polymerase Chain Reaction*
Sensitivity and Specificity
Triose-Phosphate Isomerase / genetics*
Chemical
Reg. No./Substance:
EC 5.3.1.1/Triose-Phosphate Isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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