Document Detail


PCR amplification of microsatellites from single cells of Karenia brevis preserved in Lugol's iodine solution.
MedLine Citation:
PMID:  17922226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and effective protocol is described for multiplex polymerase chain reaction (PCR) amplification of single cells of Karenia brevis. The protocol requires minimum processing, avoids additions that might dilute target DNA template, and can be used on cells preserved in Lugol's iodine preservative. Destaining of Lugol's-preserved cells with sodium thiosulfate allowed successful amplification of single-copy, nuclear-encoded microsatellites in single cells of K. brevis that have been preserved for up to 6 years.
Authors:
D W Henrichs; M A Renshaw; C A Santamaria; B Richardson; J R Gold; L Campbell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-10-07
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  10     ISSN:  1436-2228     ISO Abbreviation:  Mar. Biotechnol.     Publication Date:    2008 Mar-Apr
Date Detail:
Created Date:  2008-03-06     Completed Date:  2008-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  122-7     Citation Subset:  IM    
Affiliation:
Department of Biology, Texas A and M University, College Station, Texas 77843-3578, USA. dhenrichs@bio.tamu.edu
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Dinoflagellida / genetics*
Genotype
Iodides
Microsatellite Repeats / genetics*
Polymerase Chain Reaction / veterinary*
Preservation, Biological / methods
Chemical
Reg. No./Substance:
0/Iodides; 12298-68-9/Lugol's solution

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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