Document Detail

PC12 neurite regeneration and long-term maintenance in the absence of exogenous nerve growth factor in response to contact with Schwann cells.
MedLine Citation:
PMID:  3986579     Owner:  NLM     Status:  MEDLINE    
We have investigated mouse and rat ganglionic Schwann cells as possible sources of neurite outgrowth-promoting factors by co-culturing Schwann cells with nerve growth factor (NGF)-responsive PC12 pheochromocytoma cells primed by pretreatment with NGF. NGF-primed PC12 cells are capable of neurite regeneration when provided with an appropriate neurite promoting factor such as NGF. When primed PC12 cells were co-cultured with Schwann cells in the absence of exogenous NGF, PC12 cells that directly contacted Schwann cells became enlarged and flattened, attaining a neuron-like morphology within one day. When contact with Schwann cells was established, PC12 cells regenerated neurites by the first day of co-culture and these were maintained throughout the experiments (7 weeks). Most PC12 cells cultured in the same collagen-coated dishes with Schwann cells, but not directly in contact with them, failed to regenerate neurites. Instead, they began to proliferate, forming cell clusters. Neurite regeneration by PC12 cells in contact with Schwann cells was not blocked by antibody to NGF. These results demonstrate the presence of a neurite-promoting activity localized to the vicinity of the Schwann cell surface which is capable of eliciting regeneration and long-term maintenance of PC12 neurites in the absence of exogenous NGF. This activity does not appear to be due to NGF.
M Cochran; M M Black
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  349     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-06-12     Completed Date:  1985-06-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  105-16     Citation Subset:  IM    
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MeSH Terms
Animals, Newborn
Cell Line
Cells, Cultured
Microscopy, Electron
Nerve Growth Factors / physiology
Nerve Tissue Proteins / physiology*
Schwann Cells / physiology*
Grant Support
Reg. No./Substance:
0/Nerve Growth Factors; 0/Nerve Tissue Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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