Document Detail


PC12 and HEL cell communication in a reconstituted synapse analyzed with mathematical modeling.
MedLine Citation:
PMID:  15567518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A synapse simulating model comprising of the nerve growth factor (NGF)-differentiated PC12 cells releasing neurotransmitter (NT) and sensor 92.1.7.human erythroleukemia (HEL) cells has been used for simulating the connection between neurons and target cells. A Ca(2+) elevation was observed in both cell types when the PC12 cells were challenged with nicotine. The response patterns of individual cell were subsequently analyzed mathematically. The Ca(2+) signals of the PC12 cells were described by an equation representing a simple bi-exponential function. The NT-noradrenaline discharged by the PC12 cells in response to nicotine caused heterogeneous secondary Ca(2+) elevations in the HEL cells after a certain delay. Model fitting of this response disclosed slow "hidden" oscillations and heterogeneous secondary Ca(2+) signals could be grouped on the basis of the oscillation frequency. As determined in control experiments with noradrenaline (NA), the value of oscillation frequency also revealed a good correlation with the NT concentration.
Authors:
Jelena Krjukova; Vladimir Mirtov; Karl E Akerman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemistry international     Volume:  46     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-11-29     Completed Date:  2005-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  83-91     Citation Subset:  IM    
Affiliation:
Division of Cell Physiology, Department of Neuroscience, Box 572, BMC, Uppsala University, Husargatan 3, 75123 Uppsala, Sweden. elena.krukova@fysiologi.uu.se
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Calcium / metabolism
Cell Communication / physiology*
Humans
Leukemia, Erythroblastic, Acute / pathology*
Microscopy, Fluorescence
Models, Neurological
Models, Statistical
Nerve Growth Factor / pharmacology
Nicotine / pharmacology
Nicotinic Agonists / pharmacology
Norepinephrine / metabolism
PC12 Cells
Rats
Synapses / physiology*
Chemical
Reg. No./Substance:
0/Nicotinic Agonists; 51-41-2/Norepinephrine; 54-11-5/Nicotine; 7440-70-2/Calcium; 9061-61-4/Nerve Growth Factor

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