Document Detail

PAX3-FOXO1 induces cannabinoid receptor 1 to enhance cell invasion and metastasis.
MedLine Citation:
PMID:  22037868     Owner:  NLM     Status:  Publisher    
Alveolar rhabdomyosarcoma (ARMS) is a muscle-derived childhood tumor characterized by production of oncogenic PAX3/7-FOXO1 chimeric transcription factors. While downstream targets of the PAX3-FOXO1 oncoprotein in ARMS have been defined, the functional relevance of these targets is unclear. Here we show that up-regulation of the cannabinoid receptor 1 (Cnr1/Cb1) by PAX3-FOXO1 in mouse primary myoblasts and ARMS cell lines, contributes to PAX3-FOXO1 phenotypes, both in vivo and in vitro. In primary myoblasts, Cnr1 was dispensable for PAX3-FOXO1 to mediate cell proliferation, differentiation or transformation; however, Cnr1 function was essential to increase the invasive capacity conferred by PAX3-FOXO1 over-expression in these cells. Genetic or pharmacological abrogation of Cnr1 inhibited the enhanced basement membrane invasion induced by PAX3-FOXO1. Cnr1 loss by either route also dramatically reduced lung metastasis formation. Taken together, our findings strongly implicate Cnr1 as a novel tractable target to inhibit ARMS invasion and metastasis.
Amy D Marshall; Irina Lagutina; Gerard C Grosveld
Related Documents :
2087268 - Discharge induction in molluscan peptidergic cells requires a specific set of autoexcit...
22104348 - High-frequency stimulation of the temporoammonic pathway induces input-specific long-te...
22210898 - Gis is required for trichome branching through ga signalling in arabidopsis.
21909398 - Trypan blue dye enters viable cells incubated with the pore-forming toxin hlyii of baci...
837918 - Effect of 2-deoxy-d-glucose on the cell-surface glycoproteins of hamster fibroblasts,.
6823098 - Sequential analysis of hepatic carcinogenesis. regeneration of liver after carbon tetra...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-28
Journal Detail:
Title:  Cancer research     Volume:  -     ISSN:  1538-7445     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Dept of Genetics, St Jude Children's Research Hosp.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Short-term nanostructural effects of high radiofrequency treatment on the skin tissues of rabbits.
Next Document:  Phosphorylation of carbonic anhydrase IX controls its ability to mediate extracellular acidification...