| PARP-1 suppresses adiponectin expression through poly(ADP-ribosyl)ation of PPAR gamma in cardiac fibroblasts. | |
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MedLine Citation:
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PMID: 18815186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Our aim was to explore the mechanism underlying the transcriptional regulation of adiponectin and its receptors (AdipoR) in cultured rat cardiac fibroblasts. METHODS AND RESULTS: Using western blot and real-time RT-PCR assays, the expression of adiponectin and its receptors was determined. Using Southwestern blot and electrophoretic mobility shift assays, the DNA binding activity of peroxisome proliferator activated receptor gamma (PPAR gamma) was determined. The results showed that adiponectin and AdipoR1 were highly expressed in cultured rat cardiac fibroblasts. Inhibition of poly(ADP-ribose) polymerase 1 (PARP-1) by 3-aminobenzamide, PJ34, or PARP-1 siRNA markedly increased the transcription of adiponectin and AdipoR1 in cultured fibroblasts, mature 3T3 L1 adipocytes, rat myocardium, and white adipose tissue. PPAR gamma was poly(ADP-ribosyl)ated by PARP-1 in cardiac fibroblasts under basal conditions. Poly(ADP-ribosyl)ation of PPAR gamma prevented its binding to DNA. Inhibition of PARP-1 enhanced the DNA binding and transactivation of PPAR gamma and increased the transcription of PPAR gamma-target genes including CD36, lipoprotein lipase, and leptin in cultured fibroblasts. CONCLUSION: PARP-1 inhibits adiponectin and AdipoR1 expression as well as PPAR gamma transactivation through poly(ADP-ribosyl)ation of PPAR gamma in cultured rat cardiac fibroblasts. |
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Authors:
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Dan Huang; Chongzhe Yang; Yan Wang; Yuhua Liao; Kai Huang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-24 |
Journal Detail:
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Title: Cardiovascular research Volume: 81 ISSN: 1755-3245 ISO Abbreviation: Cardiovasc. Res. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-04-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: England |
Other Details:
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Languages: eng Pagination: 98-107 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Institute of Cardiovascular Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate Ribose
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metabolism* Adiponectin / metabolism* Animals Antigens, CD36 / metabolism Benzamides / pharmacology Cells, Cultured Enzyme Inhibitors / pharmacology Fibroblasts / cytology, metabolism* Heart Ventricles / cytology, metabolism* Leptin / metabolism Lipoprotein Lipase / metabolism Male PPAR gamma / metabolism* Phenanthrenes / pharmacology Poly(ADP-ribose) Polymerases / antagonists & inhibitors, metabolism* RNA, Messenger / metabolism Rats Rats, Wistar Receptors, Adiponectin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Antigens, CD36; 0/Benzamides; 0/Enzyme Inhibitors; 0/Leptin; 0/N-(oxo-5,6-dihydrophenanthridin-2-yl)-N,N-dimethylacetamide hydrochloride; 0/PPAR gamma; 0/Phenanthrenes; 0/RNA, Messenger; 0/Receptors, Adiponectin; 0/adiponectin receptor 1, rat; 20762-30-5/Adenosine Diphosphate Ribose; 3544-24-9/3-aminobenzamide; EC 2.4.2.30/Adprt protein, rat; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.1.1.34/Lipoprotein Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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