Document Detail

PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains.
MedLine Citation:
PMID:  16510507     Owner:  NLM     Status:  MEDLINE    
Epithelial cysts are one of the fundamental architectures for mammalian organogenesis. Although in vitro studies using cultured epithelial cells have revealed proteins required for cyst formation, the mechanisms that orchestrate the functions of these proteins in vivo remain to be clarified. We show that the targeted disruption of the mouse Par3 gene results in midgestational embryonic lethality with defective epicardial development. The epicardium is mainly derived from epicardial cysts and essential for cardiomyocyte proliferation during cardiac morphogenesis. PAR3-deficient epicardial progenitor (EPP) cells do not form cell cysts and show defects in the establishment of apical cortical domains, but not in basolateral domains. In PAR3-deficient EPP cells, the localizations of aPKC, PAR6beta and ezrin to the apical cortical domains are disturbed. By contrast, ZO1 and alpha4/beta1 integrins normally localize to cell-cell junctions and basal domains, respectively. Our observations indicate that EPP cell cyst formation requires PAR3 to interpret the polarity cues from cell-cell and cell-extracellular matrix interactions so that each EPP cell establishes apical cortical domains. These results also provide a clear example of the proper organization of epithelial tissues through the regulation of individual cell polarity.
Tomonori Hirose; Mika Karasawa; Yoshinobu Sugitani; Masayoshi Fujisawa; Kazunori Akimoto; Shigeo Ohno; Tetsuo Noda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-01
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  133     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-10     Completed Date:  2006-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1389-98     Citation Subset:  IM    
Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama 236-0004, Japan.
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MeSH Terms
Blotting, Western
Cell Polarity*
Cysts / metabolism*
Fluorescent Antibody Technique, Indirect
Fluorescent Dyes
Gene Targeting
Mice, Inbred C57BL
Microscopy, Fluorescence
Models, Biological
Organ Culture Techniques
Pericardium / cytology,  growth & development*,  pathology
Receptors, Thrombin / genetics*,  physiology*
Restriction Mapping
Reg. No./Substance:
0/Fluorescent Dyes; 0/Receptors, Thrombin; 0/protease-activated receptor 3; 2321-07-5/Fluorescein

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