Document Detail

PACAP ameliorates oxidative stress in the chicken inner ear: an in vitro study.
MedLine Citation:
PMID:  19969027     Owner:  NLM     Status:  MEDLINE    
Pituitary adenylate cyclase activating polypeptide (PACAP) is a pleiotropic and multifunctional neuropeptide. Numerous studies prove that PACAP has neuroprotective effects in diverse neuronal systems in vitro and in vivo. The involvement of PACAP in visual and olfactory sensory processing has also been documented, but little is known about its effects in the auditory system. The presence of PACAP and its receptor, the specific PAC1 receptor, has been shown in the cochlea and in brain structures involved in auditory pathways. The aim of the present study was to investigate whether PACAP is protective in cochlear oxidative stress-induced cell death, which is known to play a role in several ototoxic insults. Chicken cochlear cells were exposed to 1mM H(2)O(2), which resulted in a marked reduction of cell viability and a parallel increase of apoptotic and necrotic cells assessed by MTT test, annexin V/propidium iodide flow cytometry and JC-1 apoptosis assay. Co-incubation with 100nM PACAP increased cell viability and reduced the percentage of apoptotic cells. Furthermore, oxidative stress increased the activation of caspase-3, while simultaneous PACAP treatment reduced it. In summary, our present results demonstrate that PACAP effectively protects cochlear cells against oxidative stress-induced apoptotic cell death.
Boglarka Racz; Gabriella Horvath; Dora Reglodi; Balazs Gasz; Peter Kiss; Ferenc Gallyas; Balazs Sumegi; Gabor Toth; Adrienne Nemeth; Andrea Lubics; Andrea Tamas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-04
Journal Detail:
Title:  Regulatory peptides     Volume:  160     ISSN:  1873-1686     ISO Abbreviation:  Regul. Pept.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-8     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Department of Biochemistry and Medical Chemistry, University of Pecs, Hungary.
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MeSH Terms
Apoptosis / drug effects
Caspase 3 / metabolism
Cell Survival / drug effects
Cells, Cultured
Cochlea / cytology,  drug effects*
Enzyme Activation / drug effects
Flow Cytometry
Oxidative Stress / drug effects*
Pituitary Adenylate Cyclase-Activating Polypeptide / pharmacology*
Signal Transduction
Reg. No./Substance:
0/Pituitary Adenylate Cyclase-Activating Polypeptide; EC 3.4.22.-/Caspase 3

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