Document Detail

P53 mutation and c-fos overexpression are associated with detection of the antigen VLA-2 in human melanoma cell lines.
MedLine Citation:
PMID:  7886007     Owner:  NLM     Status:  MEDLINE    
In this study, we examined the expression of c-fos, c-myc, mutant c-Ha-ras and mutant p53 proteins in three normal human melanocyte cell lines and the following 12 melanoma cell lines: M5, Mewo, A375, Bro, Mel 2a, O-Mel II, IgR 39, SkMel-13, -19, -28 Mel-57 and NKI-4, using an immunohistochemical assay (APAAP). An effort was made to correlate oncogene expression with growth parameters, differentiation antigens (HMB-45, vla-2, k.1.2.58, HLA-DR, HLA-I), and pigmentation. All melanocyte cell lines were negative for the oncogenes examined, whereas six of the melanoma cell lines were found also positive (three for c-fos, two for c-myc, one for c-Ha-ras, and four for p53). Three melanoma cell lines expressed one oncogene and three the combination c-fos/p53. These three melanoma cell lines were positive for the "late" tumor progression marker A. 1.43 (vla-2 adhesion molecule) and negative for the differentiation marker k. 1. 2. 58. Positivity for A. 1. 43 combined with negative staining for k. 1. 2. 58 was found in six out of the 12 cell lines. The observed oncogene expression correlated neither with growth parameters nor melanin content. The present findings revealed a coexpression of mutant p53 and c-fos proteins being associated with a highly malignant phenotype in melanoma cell lines. Further studies are necessary to clarify the significance of the above findings.
G Kroumpouzos; J Eberle; C Garbe; C E Orfanos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society     Volume:  7     ISSN:  0893-5785     ISO Abbreviation:  Pigment Cell Res.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1995-04-13     Completed Date:  1995-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800247     Medline TA:  Pigment Cell Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  348-53     Citation Subset:  IM    
Department of Dermatology, University Medical Center Steglitz, Free University of Berlin, Germany.
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MeSH Terms
Gene Expression*
Genes, fos*
Genes, p53*
Melanoma / genetics*,  immunology
Proto-Oncogene Proteins c-fos / analysis
Receptors, Very Late Antigen / analysis*
Tumor Cells, Cultured
Tumor Markers, Biological / analysis
Tumor Suppressor Protein p53 / analysis
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Receptors, Very Late Antigen; 0/Tumor Markers, Biological; 0/Tumor Suppressor Protein p53

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