| P2Y2 receptor transcription is increased by NF-kappa B and stimulates cyclooxygenase-2 expression and PGE2 released by intestinal epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 19734210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Inflammatory stresses associated with inflammatory bowel diseases up-regulate P2Y(2) mRNA receptor expression in the human colon adenocarcinoma cell line Caco-2, the noncancerous IEC-6 cells and in colonic tissues of patient suffering from Crohn's disease and ulcerative colitis. However, the transcriptional events regulating P2Y(2) receptor (P2Y(2)R) expression are not known. We have identified a putative transcription start site in the P2Y(2)R gene and demonstrated acetylation of Lys(14) on histone H3 and Lys(8) on histone H4, thus suggesting that the chromatin associated with the P2Y(2) promoter is accessible to transcription factors. We also showed that the transcription factor NF-kappaB p65 regulates P2Y(2)R transcription under both proinflammatory and basal conditions. A NF-kappaB-responsive element was identified at -181 to -172 bp in the promoter region of P2Y(2). Hence, activation of P2Y(2)R by ATP and UTP stimulated cyclooxygenase-2 expression and PGE(2) secretion by intestinal epithelial cells. These findings demonstrate that P2Y(2)R expression is regulated during intestinal inflammation through an NF-kappaB p65-dependent mechanism and could contribute not only to inflammatory bowel disease but also to other inflammatory diseases by regulating PG release. |
| | |
Authors:
|
Emilie Degagné; Djordje M Grbic; Andrée-Anne Dupuis; Elise G Lavoie; Christine Langlois; Nishant Jain; Gary A Weisman; Jean Sévigny; Fernand-Pierre Gendron |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-09-04 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 183 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-21 Completed Date: 2009-11-16 Revised Date: 2011-09-22 |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 4521-9 Citation Subset: AIM; IM |
Affiliation:
|
Canadian Institutes of Health Research Team on the Digestive Epithelium, Département d'Anatomie et de Biologie Cellulaire, Université de Sherbrooke, Sherbrooke, Québec, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Caco-2 Cells Cell Line Cyclooxygenase 2 / biosynthesis*, genetics Dinoprostone / secretion* Humans Inflammation Mediators / physiology Intestinal Mucosa / cytology, enzymology, pathology, secretion* Promoter Regions, Genetic / immunology Rats Receptors, Purinergic P2 / biosynthesis, genetics*, physiology Receptors, Purinergic P2Y2 Transcription Factor RelA / physiology* Transcription, Genetic / immunology* Up-Regulation / genetics*, immunology* |
| Grant Support | |
ID/Acronym/Agency:
|
67520//Canadian Institutes of Health Research; 68957//Canadian Institutes of Health Research; 93683//Canadian Institutes of Health Research; AG18357/AG/NIA NIH HHS; DE07389/DE/NIDCR NIH HHS; DE17591/DE/NIDCR NIH HHS; R01 DE007389-21A1/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Inflammation Mediators; 0/P2RY2 protein, human; 0/Receptors, Purinergic P2; 0/Receptors, Purinergic P2Y2; 0/Transcription Factor RelA; 363-24-6/Dinoprostone; EC 1.14.99.1/Cyclooxygenase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Toll-IL-1R member Tir8/SIGIRR negatively regulates adaptive immunity against kidney grafts.
Next Document: Fluid flow regulates stromal cell organization and CCL21 expression in a tissue-engineered lymph nod...