Document Detail


P2X7 receptor modulation of the viability of radial glial clone L2.3 cells during hypoxic-ischemic brain injury.
MedLine Citation:
PMID:  22377955     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purinergic P2X7 receptor (P2X7R) can be activated by ATP and plays significant and complex roles in neuropathology. However, research is limited concerning the role of P2X7R in radial glia following hypoxia-ischemia (HI). In this study, radial glial clone L2.3 cells were cultured and subjected to oxygen-glucose deprivation (OGD) to generate an HI model in vitro. We found that HI decreased P2X7R expression in the L2.3 cells. Activation of P2X7R in L2.3 cells by 3'-O-(4-benzoylbenzoyl) adenosine 5'-triphosphate (BzATP) led to cell death in a dose- and time-dependent manner, while a P2X7R antagonist, oxidized ATP (oATP), alleviated the injury induced by BzATP or HI. We also found that P2X7R modulated the phosphorylation of glycogen synthase kinase-3β (GSK-3β). The present findings suggest that L2.3 cells express P2X7R, and this receptor may be involved in HI injury of radial glia by mediating phosphorylation of GSK-3β.
Authors:
Wen Zeng; Yu Tong; Hedong Li; Rong Luo; Meng Mao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-29
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-03-12     Completed Date:  2012-07-03     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1357-61     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analogs & derivatives,  metabolism,  pharmacology
Cell Hypoxia
Cell Line
Gene Expression Regulation*
Glucose / metabolism
Glycogen Synthase Kinase 3 / metabolism
Humans
Hypoxia-Ischemia, Brain / metabolism*,  pathology
Models, Biological
Neuroglia / metabolism*,  pathology
Oxygen
Phosphorylation / drug effects
Receptors, Purinergic P2X7 / biosynthesis*
Chemical
Reg. No./Substance:
0/Receptors, Purinergic P2X7; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate; 7782-44-7/Oxygen; EC 2.7.11.1/glycogen synthase kinase 3 beta; EC 2.7.11.26/Glycogen Synthase Kinase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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