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P19 embryonal carcinoma cells exhibit high sensitivity to botulinum type C and D/C mosaic neurotoxins.
MedLine Citation:
PMID:  22738015     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Botulinum neurotoxins (BoNTs) inhibit neurotransmitter release at peripheral nerve terminals. They are serologically classified from A to G, with C/D and D/C mosaic neurotoxins forming further subtypes of serotypes C and D. Cultured primary neurons as well as neuronal cell lines such as PC12 and Neuro-2a are often utilized in cell-based experiments for the toxic action of botulinum toxins. However, there are very few reports of the use of neural cell lines for studying BoNTs/C and D. In addition, the differentiated P19 neuronal cell line, which possesses cholinergic properties, has yet to be tested for its susceptibility to BoNTs. Here, we report the responsiveness of differentiated P19 cells to BoNT/C and BoNT/DC. Both BoNT/C and BoNT/DC were shown to effectively bind to and be internalized by neurons derived from P19 cells. Subsequently, the intracellular substrates for BoNT/C and BoNT/DC were cleaved by treatment of the cells with the toxins in a ganglioside-dependent manner. Moreover, P19 neurons exhibited high sensitivity to BoNT/C and BoNT/DC, to the same extent as cultured primary neurons. These findings suggest that differentiated P19 cells possess full sensitivity to BoNT/C and BoNT/DC, thus making them a novel susceptible cell line for research into BoNTs.
Authors:
Kentaro Tsukamoto; Hideyuki Arimitsu; Sadayuki Ochi; Keiji Nakamura; Yoshikazu Tanaka; Nipawan Nuemket; Koki Taniguchi; Shunji Kozaki; Takao Tsuji
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-27
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan Creative Research Institution "Sousei", Hokkaido University, Sapporo 001-0021, Japan Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan Graduate School of Life Sciences, Hokkaido University, Sapporo 060-0810, Japan Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
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