Document Detail


P1 nuclease cleavage is dependent on length of the mismatches in DNA.
MedLine Citation:
PMID:  18524693     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
P1 nuclease is one of the most extensively used single-strand DNA specific nucleases in molecular biology. In modern biology, it is used as an enzymatic probe to detect altered DNA conformations. It is well documented that P1 cleaves single-stranded nucleic acids and single-stranded DNA regions. The fact that P1 can act under a wide range of conditions, including physiological pH and temperature make it the most commonly used enzymatic probe in DNA structural studies. Surprisingly, to this date, there is no study to characterize the influence of length of mismatches on P1 sensitivity. Using a series of radioactively labeled oligomeric DNA substrates-containing mismatches, we find that P1 nuclease cleavage is dependent on the length of mismatches. P1 does not cleave DNA when there is a single-base mismatch. P1 cleavage efficiency is optimum when mismatch length is 3 nt or more. Changing the position of the mismatches also does not make any difference in cleavage efficacy. These novel findings on P1 properties have implications for its use in DNA structure and DNA repair studies.
Authors:
Abani Kanta Naik; Sathees C Raghavan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-04
Journal Detail:
Title:  DNA repair     Volume:  7     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-10-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1384-91     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Indian Institute of Science, Bangalore, India.
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MeSH Terms
Descriptor/Qualifier:
Base Pair Mismatch*
Base Sequence
DNA Primers
Deoxyribonucleases / metabolism*
Genomic Instability
Hydrolysis
Potassium Permanganate / chemistry
Chemical
Reg. No./Substance:
0/DNA Primers; 7722-64-7/Potassium Permanganate; EC 3.1.-/Deoxyribonucleases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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