Document Detail

P. nalgiovense carries a gene which is homologous to the paf gene of P. chrysogenum which codes for an antifungal peptide.
MedLine Citation:
PMID:  11139027     Owner:  NLM     Status:  MEDLINE    
Penicillium nalgiovense is related to P. chrysogenum. P. chrysogenum carries the paf gene (Penicillium antifungal peptide) with homology to the afp gene of Aspergillus giganteus. This gene codes for a peptide with antifungal activity. Based on the sequence of the published P. chrysogenum paf gene primers were generated. By the use of these primers a PCR product of the expected length could be isolated from strains of P. nalgiovense. This fragment was sequenced and compared to the sequence of the paf gene of P. chrysogenum. According to the results P. nalgiovense carries a gene (naf = P. nalgiovense antifungal peptide) which is highly homologous to the paf gene of P. chrysogenum. The gene also codes for a preproprotein with the same processing sites as the paf gene, suggesting that the mature product is also a 55 amino acid (aa) peptide. The naf gene has three amino acid exchanges compared to the paf gene, which however do not influence the amino acid sequence of the mature peptide. It also carries two introns at the same positions, however, the sequence differences between the introns are higher than between the coding regions. When P. nalgiovense were grown on plates containing other food-relevant fungi it showed weak antifungal activity.
R Geisen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of food microbiology     Volume:  62     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-02     Completed Date:  2001-07-19     Revised Date:  2003-02-24    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  95-101     Citation Subset:  IM    
Federal Research Centre for Nutrition, Karlsruhe, Germany.
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MeSH Terms
Antifungal Agents / metabolism
Base Sequence
Blotting, Southern
DNA Primers
DNA, Fungal / chemistry*,  isolation & purification
Fungal Proteins / biosynthesis,  genetics*
Gene Expression Regulation, Fungal
Molecular Sequence Data
Penicillium / genetics*,  metabolism
Penicillium chrysogenum / genetics,  metabolism
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
Reg. No./Substance:
0/Antifungal Agents; 0/DNA Primers; 0/DNA, Fungal; 0/Fungal Proteins; 0/PAF protein, Penicillium chrysogenum

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