Document Detail


P-glycoprotein (multi-xenobiotic resistance) and heat shock protein gene expression in the reef coral Montastraea franksi in response to environmental toxicants.
MedLine Citation:
PMID:  19501419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The deleterious impacts of marine pollutants on reef corals and their symbiotic algae are an important element of global coral reef decline. In the current study we examined the impacts of toxicants on the reef coral Montastraea franksi by analysing the expression of three stress-related genes belonging to the coral host, using Taqman real-time quantitative reverse transcription-PCR. Gene expression profiles of P-glycoprotein (or multi-xenobiotic resistance protein) (P-gp); heat shock protein 70 (Hsp70) and heat shock protein 90 (Hsp90) were examined following 4 and 8h exposures to the heavy metal copper (3, 10, 30 and 100 microgL(-1)) or the third generation oil dispersant Corexit9527 (1, 5, 10 and 50 ppm). Additionally, the expression of P-gp was examined following exposure to 0.5 and 5 microM concentrations of the chemotherapeutic drug vinblastine, a classic substrate of P-gp. The expression of P-gp increased significantly in corals treated with vinblastine and also increased following exposure to copper and Corexit9527. Hsp70, and to a lesser extent Hsp90 expression increased following exposure to copper and Corexit9527 indicating a general cellular stress response. Densities of symbiotic algae in the tissues of the corals did not change significantly during the experiments, nor was any loss or paling of coral tissues observed. These findings provide important insight into how corals defend themselves against pollution and complement ongoing initiatives developing molecular biomarkers of stress in reef-building corals.
Authors:
Alexander A Venn; Jennifer Quinn; Ross Jones; Andrea Bodnar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-09
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  93     ISSN:  1879-1514     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-08     Completed Date:  2009-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  188-95     Citation Subset:  IM    
Affiliation:
Bermuda Institute of Ocean Sciences (BIOS), Ferry Reach, St. Georges, GE 01, Bermuda. alex@centrescientifique.mc
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MeSH Terms
Descriptor/Qualifier:
Algae / growth & development
Animals
Anthozoa / drug effects,  genetics*,  metabolism
Copper / toxicity
DNA, Complementary / isolation & purification
Gene Expression / drug effects
Gene Expression Profiling
HSP70 Heat-Shock Proteins / drug effects,  genetics,  metabolism
HSP90 Heat-Shock Proteins / drug effects,  genetics,  metabolism
Heat-Shock Proteins / drug effects,  genetics*,  metabolism
Lipids / toxicity
P-Glycoprotein / drug effects,  genetics*,  metabolism
Surface-Active Agents / toxicity
Vinblastine / toxicity
Water Pollutants, Chemical / toxicity*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/HSP70 Heat-Shock Proteins; 0/HSP90 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Lipids; 0/P-Glycoprotein; 0/Surface-Active Agents; 0/Water Pollutants, Chemical; 60617-06-3/corexit 9527; 7440-50-8/Copper; 865-21-4/Vinblastine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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