Document Detail


P. gingivalis lipopolysaccharide intensifies inflammation post-myocardial infarction through matrix metalloproteinase-9.
MedLine Citation:
PMID:  25240641     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Periodontal disease (PD) strongly correlates with increased mortality post-myocardial infarction (MI); however, the underlying mechanisms are unknown. Matrix metalloproteinase (MMP)-9 levels directly correlate with dysfunction and remodeling of the left ventricle (LV) post-MI. Post-MI, MMP-9 is produced by leukocytes and modulates inflammation. We have shown that exposure to Porphyromonas gingivalis lipopolysaccharide (PgLPS), an immunomodulatory molecule identified in PD patients, increases LV MMP-9 levels in mice and leads to cardiac inflammation and dysfunction. The aim of the study was to determine if circulating PgLPS exacerbates the LV inflammatory response post-MI through MMP-9 dependent mechanisms. We exposed wild type C57BL/6J and MMP-9(-/-) mice to PgLPS (ATCC 33277) for a period of 28days before performing MI, and continued to deliver PgLPS for up to 7days post-MI. We found systemic levels of PgLPS 1) increased MMP-9 levels in both plasma and infarcted LV resulting in reduced wall thickness and increased incidence of LV rupture post-MI and 2) increased systemic and local macrophage chemotaxis leading to accelerated M1 macrophage infiltration post-MI and decreased LV function. MMP-9 deletion played a protective role by attenuating the inflammation induced by systemic delivery of PgLPS. In conclusion, MMP-9 deletion has a cardioprotective role against PgLPS exposure, by attenuating macrophage mediated inflammation.
Authors:
Kristine Y DeLeon-Pennell; Lisandra E de Castro Brás; Rugmani Padmanabhan Iyer; Dustin R Bratton; Yu-Fang Jin; Crystal M Ripplinger; Merry L Lindsey
Publication Detail:
Type:  Journal Article     Date:  2014-09-18
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  76     ISSN:  1095-8584     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-12-02     Completed Date:  -     Revised Date:  2014-12-03    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  218-26     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 Elsevier Ltd. All rights reserved.
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Grant Support
ID/Acronym/Agency:
HHSN268201000036C/HL/NHLBI NIH HHS; P01 HL051971/HL/NHLBI NIH HHS; P20 GM104357/GM/NIGMS NIH HHS; R01 HL075360/HL/NHLBI NIH HHS; R01 HL111600/HL/NHLBI NIH HHS

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