Document Detail

P element excision and repair by non-homologous end joining occurs in both G1 and G2 of the cell cycle.
MedLine Citation:
PMID:  15590325     Owner:  NLM     Status:  MEDLINE    
P element excision generates a DNA double-strand break at the transposon donor site. Genetic studies have demonstrated a strong bias toward repair of P element-induced DNA breaks by homologous recombination with the sister chromatid, suggesting that P element excision occurs after DNA replication, in G2 of the cell cycle. We developed methods to arrest Drosophila tissue culture cells and assay P element excision in either G1- or G2-arrested cells. Dacapo or tribbles transgene expression arrests cells in either G2 or G2, respectively. RNA-mediated gene interference (RNAi) directed against cyclin E or cyclin A arrests cells in G1 or G2, respectively. P element excision occurs efficiently in both G1- and G2-arrested cells, suggesting that cell cycle regulation of P element transposase does not occur in our somatic cell system. DNA double-strand break repair occurs by two predominant mechanisms: homologous recombination (HR) and non-homologous end joining (NHEJ). HR is thought to be restricted to the post-replicative, G2, phase of the cell cycle, while NHEJ may occur throughout the cell cycle. Our results indicate that NHEJ repair of an extrachromasomal plasmid substrate occurs at least as efficiently in G2-arrested cells as in asynchronous cells or in G1-arrested cells.
Brian T Weinert; Bosun Min; Donald C Rio
Related Documents :
18064355 - The chromatin structure of the lysozyme gas41 origin of dna replication changes during ...
15715935 - Antisense oligodeoxynucleotides targeting the serine/threonine kinase pim-2 inhibited p...
12065055 - Roles of dna-dependent protein kinase and atm in cell-cycle-dependent radiation sensiti...
10660075 - Slg1 plays a role during g1 in the decision to enter or exit the cell cycle.
15603925 - Toxic and hormonal effects of polychlorinated biphenyls on cultured testicular germ cel...
11846445 - Glyceraldehyde-3-phosphate dehydrogenase associates with actin filaments in serum depri...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  DNA repair     Volume:  4     ISSN:  1568-7864     ISO Abbreviation:  DNA Repair (Amst.)     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-05-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  101139138     Medline TA:  DNA Repair (Amst)     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  171-81     Citation Subset:  IM    
Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California, Berkeley, 16 Barker Hall, CA 94720-3204, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle Proteins / physiology
DNA Damage
DNA Repair*
DNA Replication
DNA Transposable Elements / genetics*
Drosophila Proteins / physiology
Drosophila melanogaster / genetics*
G1 Phase / genetics*
G2 Phase / genetics*
Nuclear Proteins / physiology
Protein-Serine-Threonine Kinases / physiology
Recombination, Genetic*
Transgenes / physiology*
Grant Support
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA Transposable Elements; 0/Dacapo protein, Drosophila; 0/Drosophila Proteins; 0/Nuclear Proteins; 0/tribbles protein, Drosophila; 9007-49-2/DNA; EC Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The yeast Snm1 protein is a DNA 5'-exonuclease.
Next Document:  Proliferation-dependent expression of nuclear uracil-DNA glycosylase is mediated in part by E2F-4.