Document Detail


Oxyphil cell function in secondary parathyroid hyperplasia.
MedLine Citation:
PMID:  8856255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxyphil cell function in secondary parathyroid hyperplasia due to chronic renal failure was evaluated using in situ hybridization and heterotransplantation of parathyroid tissue. In situ hybridization and histologic analysis were performed on continuous frozen sections using 22 parathyroid tissues. A restricted area composed exclusively of oxyphil cells was observed in 10 specimens, and an area of only chief cells was found in 12 specimens. Silver grains demonstrating the existence of parathyroid hormone (PTH) mRNA were 18.8 +/- 7.8 (mean +/- SD) in oxyphil cells while those in chief cells were 17.2 +/- 7.5. PTH mRNA was abundant in both the oxyphil and chief cells. Further analysis of oxyphil cell function was assessed by the heterotransplantation of parathyroid nodules, consisting exclusively of oxyphil or chief cells, into nude mice. The function of these implants was assessed by measuring the concentration of human intact PTH which did not cross-react with mouse PTH. Serum PTH concentrations were correlated with the volume of implanted tissue. Elevations of PTH concentrations were similar in the mice transplanted with oxyphil or chief cells, indicating that both cell types had similar PTH secretory activity. The basic histologic characteristics of both cell types were not altered following transplantation. These results demonstrate that oxyphil cells in secondary parathyroid hyperplasia synthesize and secrete PTH, and that this secretion contributes to the pathophysiology of hyperparathyroidism.
Authors:
Y Tanaka; H Funahashi; T Imai; H Seo; Y Tominaga; H Takagi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nephron     Volume:  73     ISSN:  0028-2766     ISO Abbreviation:  Nephron     Publication Date:  1996  
Date Detail:
Created Date:  1997-01-03     Completed Date:  1997-01-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0331777     Medline TA:  Nephron     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  580-6     Citation Subset:  IM    
Affiliation:
Department of Surgery II, Nagoya University School of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Animals
Calcium / metabolism
Cell Transplantation
Eosinophils / physiology*
Female
Humans
Hyperparathyroidism / pathology*
Hyperplasia
In Situ Hybridization
Mice
Mice, Nude
Parathyroid Glands / pathology*,  transplantation
Parathyroid Hormone / biosynthesis
Phosphorus / metabolism
RNA, Messenger / biosynthesis
Transplantation, Heterologous
Chemical
Reg. No./Substance:
0/Parathyroid Hormone; 0/RNA, Messenger; 7440-70-2/Calcium; 7723-14-0/Phosphorus; EC 3.1.3.1/Alkaline Phosphatase

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