Document Detail


Oxylipin and mitochondrion probes to track yeast sexual cells.
MedLine Citation:
PMID:  18535630     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests increased mitochondrial activity in asci, since 3-OH oxylipins are mitochondrially produced and it is known that Rh123 accumulates selectively in functional mitochondria that maintain a high transmembrane potential (Delta Psi m). This increased activity may be necessary for the production and effective release of the many spores found in single-celled asci. These results may be useful in the rapid identification of asci and in yeast sexual spore mechanics, which may find application in yeast systematics as well as hydro-, aero-, and nano-technologies.
Authors:
Desmond M Ncango; Chantel W Swart; Monique E Goldblatt; Carolina H Pohl; Pieter W J Van Wyk; Piet J Botes; Johan L F Kock
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  54     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-06     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  450-5     Citation Subset:  IM    
Affiliation:
UNESCO MIRCEN Industrial Biotechnology, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
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MeSH Terms
Descriptor/Qualifier:
Cytophotometry / methods*
Fluorescent Dyes / analysis,  metabolism
Microscopy, Confocal
Mitochondria / chemistry,  metabolism
Molecular Probes / analysis*,  metabolism
Oxylipins / analysis*,  metabolism
Rhodamine 123 / analysis,  metabolism
Saccharomycetales / chemistry,  cytology*,  metabolism
Spores, Fungal / chemistry,  cytology*,  metabolism
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Molecular Probes; 0/Oxylipins; 62669-70-9/Rhodamine 123

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