Document Detail


Oxygenation of phosphatidylcholine by human polymorphonuclear leukocyte 15-lipoxygenase.
MedLine Citation:
PMID:  3927906     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cell-free human polymorphonuclear leukocyte preparation containing both 15- and 5-lipoxygenase activities was found to oxygenate phosphatidylcholine at carbon-15 of the arachidonic acid moiety. No oxygenation at carbon-5 was found. Under similar incubation conditions, soybean and rabbit reticulocyte 15-lipoxygenases also oxygenated phosphatidylcholine, whereas rat basophilic leukemia cell 5-lipoxygenase, rabbit platelet 12-lipoxygenase and rat liver cytochrome P-450 preparations did not. Our results suggest that the oxygenation of phospholipids may be a unique property of the 15-lipoxygenases.
Authors:
G Jung; D C Yang; A Nakao
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  130     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1985-09-16     Completed Date:  1985-09-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  559-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonate Lipoxygenases
Arachidonic Acid
Arachidonic Acids / metabolism
Basophils / enzymology
Blood Platelets / enzymology
Cell-Free System
Cytochrome P-450 Enzyme System / metabolism
Humans
Lipoxygenase / metabolism*
Liver / enzymology
Neutrophils / enzymology*
Oxygen
Phosphatidylcholines / metabolism*
Rabbits
Rats
Species Specificity
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Phosphatidylcholines; 506-32-1/Arachidonic Acid; 7782-44-7/Oxygen; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.13.11.-/Arachidonate Lipoxygenases; EC 1.13.11.12/Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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