Document Detail


Oxygenated machine perfusion preservation of predamaged kidneys with HTK and Belzer machine perfusion solution: an experimental study in pigs.
MedLine Citation:
PMID:  16298570     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of the present study was to evaluate the recently proposed aerobic machine preservation with the noncolloidal HTK solution by comparison with the colloidal Belzer machine perfusion solution (MPS) after procurement of marginal kidneys from non-heart-beating donors. Kidneys were harvested 40 minutes after cardiac arrest in German Landrace pigs and subjected to 18 hours of oxygenated hypothermic machine perfusion with either Belzer MPS or modified HTK via the renal artery (Psys < 40 mm Hg). During machine perfusion transrenal flow was approximatively twofold higher and calculated oxygen uptake was increased by 30% using the colloidal Belzer MPS, but overall enzyme release was comparable in both groups. After heterotopic transpantation with bilateral nephrectomy of the recipient, there were no differences with respect to initial tissue perfusion in vivo (evaluated by laser Doppler flowmetry) as well as urine production and median serum levels of urea or creatinine over 1 week of follow-up between grafts perfused with HTK or Belzer MPS. In conclusion, provision of oxygen during storage is possible by low-flow perfusion with HTK as with Belzer MPS.
Authors:
S Manekeller; H Leuvenink; M Sitzia; T Minor
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article    
Journal Detail:
Title:  Transplantation proceedings     Volume:  37     ISSN:  0041-1345     ISO Abbreviation:  Transplant. Proc.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-11-21     Completed Date:  2006-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3274-5     Citation Subset:  IM    
Affiliation:
Division of Surgical Research, University Hospital of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenosine
Allopurinol
Animals
Glucose
Glutathione
Heart Arrest
Insulin
Kidney* / pathology
Kidney Function Tests
Mannitol
Models, Animal
Organ Preservation / methods*
Organ Preservation Solutions*
Perfusion
Potassium Chloride
Procaine
Raffinose
Swine
Chemical
Reg. No./Substance:
0/Bretschneider cardioplegic solution; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 50-99-7/Glucose; 512-69-6/Raffinose; 58-61-7/Adenosine; 59-46-1/Procaine; 69-65-8/Mannitol; 70-18-8/Glutathione; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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