| Oxygen transfer properties of a bioreactor for use within a nuclear magnetic resonance spectrometer. | |
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MedLine Citation:
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PMID: 18587814 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Nuclear magnetic resonance (NMR) spectroscopic analysis of whole cells is an important emerging technique for noninvasive and nondestructive monitoring of cell physiology. However, this technique requires extremely high cell densities. Attempts to maintain densities above the carrying capacity of a maintenance system result in the demise of the entire culture. To define conditions for maintaining mammalian cells at high densities for NMR studies, we have designed a bioreactor to operate under defined, oxygen-limited conditions within an NMR spectrometer. The bioreactor utilizes hollow fibers to deliver nutrients and remove wastes from an agitated cell suspension. The mass transfer properties of the fibers with respect to oxygen were determined. Ehrlich Ascites Tumor (EAT) cells were supplied with glutamine as the respiratory carbon source. The maximum viable cell density supported by a given oxygen concentration in the fluid flowing through the fiber lumen was predicted and then confirmed experimentally on the bench and in the spectrometer. |
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Authors:
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D D Drury; B E Dale; R J Gillies |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 32 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1988 Oct |
Date Detail:
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Created Date: 2008-06-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 966-74 Citation Subset: - |
Affiliation:
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Department of Agricultural and Chemical Engineering, Colorado State University, Fort Collins, Colorado 80523, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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