| Oxygen tension directs the differentiation pathway of human cytotrophoblast cells. | |
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MedLine Citation:
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PMID: 17706280 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During placental development, human cytotrophoblast cells can differentiate to either villous syncytiotrophoblast cells or invasive extravillous trophoblast cells. We hypothesize that oxygen tension plays a critical role in determining the pathway of cytotrophoblast differentiation. A highly purified preparation of cytotrophoblast cells from human third trimester placenta was cultured for 5 days in either 20% or 1% oxygen tension. The cells incubated at 20% oxygen formed a syncytium as determined by immunohistochemistry using an anti-desmosomal protein antibody that identifies cell membranes. In addition, the mRNA was markedly induced for syncytin, a glycoprotein shown to be essential for syncytiotrophoblast formation, and for human placental lactogen (hPL), which is a specific marker for syncytiotrophoblast cells. In contrast, the cell incubated at 1% oxygen tension did not fuse by morphologic analysis and did not express syncytin or hPL mRNA. However, these cells expressed abundant amounts of HLA-G, a specific marker for extravillous trophoblast cells, which was not seen in cells incubated at 20% oxygen tension. These results suggest that low oxygen tension directs differentiation along the extravillous trophoblast cell pathway while greater oxygen tension directs differentiation along the villous trophoblast cell pathway. |
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Authors:
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J C Robins; A Heizer; A Hardiman; M Hubert; S Handwerger |
Publication Detail:
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Type: Journal Article Date: 2007-08-13 |
Journal Detail:
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Title: Placenta Volume: 28 ISSN: 0143-4004 ISO Abbreviation: Placenta Publication Date: 2007 Nov-Dec |
Date Detail:
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Created Date: 2007-10-22 Completed Date: 2008-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 1141-6 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Cincinnati Medical Center, Cincinnati, OH 45267, USA. jared.robins@brown.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Differentiation
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genetics,
metabolism Cell Differentiation / drug effects, physiology* Cells, Cultured Female Gene Expression Regulation, Developmental / drug effects Giant Cells / cytology, physiology Humans Immunohistochemistry Oxygen / metabolism*, pharmacology Placenta / cytology, growth & development, physiology* Polymerase Chain Reaction Pregnancy Signal Transduction / drug effects, physiology* Time Factors Trophoblasts / cytology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Differentiation; 7782-44-7/Oxygen |
| Comments/Corrections | |
Comment In:
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Placenta. 2008 Mar;29(3):305-6; author reply 307
[PMID:
18221993
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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