| Oxygen tension correlates with regional blood flow in obstructed rat kidney. | |
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MedLine Citation:
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PMID: 19749109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As renal tissue oxygen tension (P(O(2))) is determined by the balance between oxygen supply and consumption, direct tissue P(O(2)) measurements are essential when evaluating the presence of hypoxia. The present study aimed at evaluating invasively and continuously the renal medullary and cortical tissue P(O(2)) by novel fibre-optic probes in rats subjected to acute unilateral ureteral obstruction (AUUO). In parallel, regional blood flow measurements were obtained by MRI to investigate the relationship between regional blood flow and tissue oxygen tension. The abundance of transport proteins was determined by immunoblotting. In the obstructed kidney, AUUO caused a prompt decrease in medullary tissue P(O(2)) to 60% of baseline level whereas cortical tissue P(O(2)) was unchanged. By contrast, tissue P(O(2)) slightly increased in the non-obstructed kidney. These changes developed during the first 30 min after AUUO and persisted for the 3 h observation period. Medullary blood flow declined 1.5-2 h after induction of AUUO to 61% of baseline level in the obstructed kidney. By contrast, cortical blood flow increased to 108% of baseline level in the non-obstructed kidney. Finally, the abundance of phosphorylated aquaporin 2 decreased significantly in the obstructed kidney medulla, but increased in the obstructed kidney cortex. The Na(+)/K(+)-ATPase abundance increased in the obstructed kidney medulla whereas the Na(+)/K(+)/2Cl(-) co-transporter abundance remained unchanged in the obstructed kidney. In conclusion, measurements of regional blood flow reflect tissue P(O(2)) changes during AUUO suggesting that reduced regional blood flow is a predictor of local hypoxia. Furthermore, the abundance of major transport protein is independent of tissue P(O(2)). |
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Authors:
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Anja M Jensen; Rikke N??rregaard; Sukru Oguzkan Topcu; J??rgen Fr??kiaer; Michael Pedersen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental biology Volume: 212 ISSN: 1477-9145 ISO Abbreviation: J. Exp. Biol. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-09-14 Completed Date: 2009-12-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
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Languages: eng Pagination: 3156-63 Citation Subset: IM |
Affiliation:
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The Water and Salt Research Center, Aarhus University Hospital, Skejby, DK 8200, Aarhus N, Denmark. anja.jensen@ki.au.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aquaporin 2 / metabolism Cell Hypoxia Kidney / blood supply*, physiopathology Magnetic Resonance Imaging Male Oxygen / metabolism* Rats Rats, Wistar Regional Blood Flow* Sodium-Potassium-Chloride Symporters / metabolism Sodium-Potassium-Exchanging ATPase / metabolism Ureteral Obstruction / physiopathology* |
| Chemical | |
Reg. No./Substance:
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0/Aqp2 protein, rat; 0/Aquaporin 2; 0/Sodium-Potassium-Chloride Symporters; 0/sodium-potassium chloride cotransporter 2 protein; 7782-44-7/Oxygen; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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