Document Detail


Oxygen response of the wine yeast Saccharomyces cerevisiae EC1118 grown under carbon-sufficient, nitrogen-limited enological conditions.
MedLine Citation:
PMID:  23001663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Discrete additions of oxygen play a critical role in alcoholic fermentation. However, few studies have quantitated the fate of dissolved oxygen and its impact on wine yeast cell physiology under enological conditions. We simulated the range of dissolved oxygen concentrations that occur after a pump-over during the winemaking process by sparging nitrogen-limited continuous cultures with oxygen-nitrogen gaseous mixtures. When the dissolved oxygen concentration increased from 1.2 to 2.7 μM, yeast cells changed from a fully fermentative to a mixed respirofermentative metabolism. This transition is characterized by a switch in the operation of the tricarboxylic acid cycle (TCA) and an activation of NADH shuttling from the cytosol to mitochondria. Nevertheless, fermentative ethanol production remained the major cytosolic NADH sink under all oxygen conditions, suggesting that the limitation of mitochondrial NADH reoxidation is the major cause of the Crabtree effect. This is reinforced by the induction of several key respiratory genes by oxygen, despite the high sugar concentration, indicating that oxygen overrides glucose repression. Genes associated with other processes, such as proline uptake, cell wall remodeling, and oxidative stress, were also significantly affected by oxygen. The results of this study indicate that respiration is responsible for a substantial part of the oxygen response in yeast cells during alcoholic fermentation. This information will facilitate the development of temporal oxygen addition strategies to optimize yeast performance in industrial fermentations.
Authors:
Felipe F Aceituno; Marcelo Orellana; Jorge Torres; Sebastián Mendoza; Alex W Slater; Francisco Melo; Eduardo Agosin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-15     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8340-52     Citation Subset:  IM    
Affiliation:
Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE34964
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MeSH Terms
Descriptor/Qualifier:
Carbon / metabolism
Ethanol / metabolism
Fermentation
Gene Expression Profiling
NAD / metabolism
Nitrogen / metabolism
Oxidation-Reduction
Oxidative Stress*
Oxygen / metabolism*
Saccharomyces cerevisiae / drug effects*,  metabolism*
Stress, Physiological*
Wine / microbiology*
Chemical
Reg. No./Substance:
53-84-9/NAD; 64-17-5/Ethanol; 7440-44-0/Carbon; 7727-37-9/Nitrogen; 7782-44-7/Oxygen
Comments/Corrections

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