| Oxygen-resistant multipotent embryonic carcinoma cell lines exhibit antimutator phenotypes. | |
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MedLine Citation:
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PMID: 7825058 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stable, oxygen-resistant cell lines (O2R) were isolated from P19 and P19H22 (APRT hemizygote) mouse embryonic carcinoma cells by serial exposures of increasing durations to 95% O2. Neurally differentiated progeny were also oxygen-resistant. P19O2R exhibited reduced oxygen-mediated micronucleation and a 10- to 20-fold reduction of the forward mutation rate at the HPRT locus in 20% O2. P19H22O2R cells showed reduced frequencies of colonies resistant to 2,6-diaminopurine. The modal karyotype of P19O2R was identical to that of a nonmodal karyotype present in the parental line [39,X,-Y, add(14)]. There was no evidence of enhanced resistance to ionizing radiation. We conclude that this general approach, when applied to pluripotent embryonic stem cells, has the potential to lead to the synthesis of antimutator strains of mice. |
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Authors:
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C E Ogburn; M S Turker; T J Kavanagh; C M Disteche; A C Smith; K Fukuchi; G M Martin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Somatic cell and molecular genetics Volume: 20 ISSN: 0740-7750 ISO Abbreviation: Somat. Cell Mol. Genet. Publication Date: 1994 Sep |
Date Detail:
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Created Date: 1995-02-16 Completed Date: 1995-02-16 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8403568 Medline TA: Somat Cell Mol Genet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 361-70 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Washington, Seattle 98195. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Phosphoribosyltransferase
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genetics Animals Cell Separation / methods* Embryo, Mammalian / cytology Feasibility Studies Gamma Rays Hypoxanthine Phosphoribosyltransferase / genetics Karyotyping Male Mice Mice, Inbred C3H Mutation* Oxygen / pharmacology* Tumor Cells, Cultured* / radiation effects |
| Grant Support | |
ID/Acronym/Agency:
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AG01751/AG/NIA NIH HHS; CA56383/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen; EC 2.4.2.7/Adenine Phosphoribosyltransferase; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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