Document Detail

Oxygen regulation of the cytochrome c oxidase subunit VI gene, COX6, in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  7488164     Owner:  NLM     Status:  MEDLINE    
Cytochrome c oxidase (CcO) in Saccharomyces cerevisiae is subject to intricate physiological control. Growth phase, carbon source, and oxygen level are three well recognized modulators of CcO expression. We focused on the subunit VI encoding gene, COX6, and detected unexpectedly complex oxygen regulation. We found that COX6 transcription possessed a critical threshold oxygen regulation between 0 and 2%. COX6 transcription was superinduced by elevated oxygen level up to 45%; however, superinduction was lost at 60% oxygen and above. The COX6 upstream activation region, UAS6, contains both glucose and heme responsive regions, and COX6 oxygen regulation was transduced through UAS6 by heme, as has been described for other oxygen regulated genes in yeast. We found that binding of the UAS6-domain 1 protein, BAF1, was unaltered by oxygen regulation. Nor were the alternative BAF1 complexes observed by growth in different glucose conditions formed by growth at different oxygen levels.
R M Wright; S L Simpson; B D Lanoil
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  216     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-21     Completed Date:  1995-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  676-85     Citation Subset:  IM    
Webb-Waring Institute for Biomedical Research, Denver, CO, USA.
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MeSH Terms
Base Sequence
Cloning, Molecular
DNA Probes
Electron Transport Complex IV / biosynthesis*,  genetics*
Escherichia coli
Gene Expression Regulation, Enzymologic / drug effects*
Gene Expression Regulation, Fungal / drug effects*
Macromolecular Substances
Molecular Sequence Data
Oxygen / pharmacology*
Recombinant Fusion Proteins / biosynthesis
Restriction Mapping
Saccharomyces cerevisiae / enzymology*,  genetics,  growth & development
Transcription, Genetic / drug effects
beta-Galactosidase / biosynthesis
Reg. No./Substance:
0/DNA Probes; 0/Macromolecular Substances; 0/Oligodeoxyribonucleotides; 0/Recombinant Fusion Proteins; 7782-44-7/Oxygen; EC Transport Complex IV; EC

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