Document Detail

Oxygen reduction and lipid peroxidation by iron chelates with special reference to ferric nitrilotriacetate.
MedLine Citation:
PMID:  2500058     Owner:  NLM     Status:  MEDLINE    
A certain iron chelate, ferric nitrilotriacetate (Fe3+-NTA) is nephrotoxic and also carcinogenic to the kidney in mice and rats, a distinguishing feature not shared by other iron chelates tested so far. Iron-promoted lipid peroxidation is thought to be responsible for the initial events. We examined its ability to initiate lipid peroxidation in vitro in comparison with that of other ferric chelates. Chelation of Fe2+ by nitrilotriacetate (NTA) enhanced the autoxidation of Fe2+. In the presence of Fe2+-NTA, lipid peroxidation occurred as measured by the formation of conjugated diene in detergent-dispersed linoleate micelles, and by the formation of thiobarbituric acid-reactive substances in the liposomes of rat liver microsomal lipids. Addition of ascorbic acid to Fe3+-NTA solution promoted dose-dependent consumption of dissolved oxygen, which indicates temporary reduction of iron. On reduction, Fe3+-NTA initiated lipid peroxidation both in the linoleate micelles and in the liposomes. Fe3+-NTA also initiated NADPH-dependent lipid peroxidation in rat liver microsomes. Although other chelators used (deferoxamine, EDTA, diethylenetriaminepentaacetic acid, ADP) enhanced autoxidation, reduction by ascorbic acid, or in vitro lipid peroxidation of linoleate micelles or liposomal lipids, NTA was the sole chelator that enhanced all the reactions.
S Hamazaki; S Okada; J L Li; S Toyokuni; O Midorikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  272     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-07-21     Completed Date:  1989-07-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  10-7     Citation Subset:  IM    
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
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MeSH Terms
Adenosine Diphosphate / pharmacology
Ascorbic Acid / pharmacology
Deferoxamine / pharmacology
Edetic Acid / pharmacology
Ferric Compounds / pharmacology*,  toxicity
Iron Chelating Agents / pharmacology*
Linoleic Acid
Linoleic Acids / metabolism
Lipid Peroxidation / drug effects*
Liposomes / metabolism
Membrane Lipids / metabolism
Microsomes, Liver / analysis
NADP / pharmacology
Nitrilotriacetic Acid* / analogs & derivatives*
Oxygen / metabolism*
Pentetic Acid / pharmacology
Rats, Inbred Strains
Reg. No./Substance:
0/Ferric Compounds; 0/Iron Chelating Agents; 0/Linoleic Acids; 0/Liposomes; 0/Membrane Lipids; 0/Micelles; 139-13-9/Nitrilotriacetic Acid; 16448-54-7/ferric nitrilotriacetate; 2197-37-7/Linoleic Acid; 50-81-7/Ascorbic Acid; 53-59-8/NADP; 58-64-0/Adenosine Diphosphate; 60-00-4/Edetic Acid; 67-43-6/Pentetic Acid; 70-51-9/Deferoxamine; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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