| Oxygen radicals induce poly(ADP-ribose) polymerase-dependent cell death in cytotoxic lymphocytes. | |
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MedLine Citation:
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PMID: 16751373 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cytotoxic T cells and NK cells will acquire features of apoptosis when exposed to oxygen radicals, but the molecular mechanisms underlying this phenomenon are incompletely understood. We have investigated the role of two enzyme systems responsible for execution of cell death, caspases and the poly(ADP-ribose) polymerase (PARP). We report that although human cytotoxic lymphocytes were only marginally protected by caspase inhibitors, PARP inhibitors completely protected lymphocytes from radical-induced apoptosis and restored their cytotoxic function. The radical-induced, PARP-dependent cell death was accompanied by nuclear accumulation of apoptosis-inducing factor and a characteristic pattern of large-fragment DNA degradation. It is concluded that the PARP/apoptosis-inducing factor axis is critically involved in oxygen radical-induced apoptosis in cytotoxic lymphocytes. |
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Authors:
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Fredrik B Thorén; Ana I Romero; Kristoffer Hellstrand |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 176 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-05 Completed Date: 2006-07-13 Revised Date: 2008-06-05 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 7301-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Virology, Sahlgrenska Academy, Göteborg University, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis Inducing Factor
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metabolism Cell Death / drug effects, immunology Cell Nucleus / metabolism Free Radicals / metabolism Humans Hydrogen Peroxide / pharmacology Killer Cells, Natural / cytology*, enzymology*, immunology, metabolism Macrophages / enzymology, metabolism Phenanthrenes / pharmacology Poly(ADP-ribose) Polymerases / antagonists & inhibitors, physiology* Reactive Oxygen Species / metabolism* T-Lymphocytes, Cytotoxic / cytology*, enzymology*, immunology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Inducing Factor; 0/Free Radicals; 0/N-(oxo-5,6-dihydrophenanthridin-2-yl)-N,N-dimethylacetamide hydrochloride; 0/Phenanthrenes; 0/Reactive Oxygen Species; 7722-84-1/Hydrogen Peroxide; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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