Document Detail


Oxygen pressures in the interstitial space of skeletal muscle and tumors in vivo.
MedLine Citation:
PMID:  18290314     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new Oxyphor (Oxyphor G3) has been used to selectively determine the oxygen pressure in interstitial (pericellular) spaces. Oxyphor G3 is a Pd-tetrabenzoporphyrin, encapsulated inside generation 2 poly-arylglycine (AG) dendrimer, and therefore is a true near infrared oxygen sensor, having a strong absorption band at 636nm and emission near 800nm. The periphery of the dendrimer is modified with oligoethylene glycol residues (Av. MW 350) to make the probe water soluble and biologically inert. Oxyphor G3 was injected along "tracks" in the tissue using a small needle (30gage or less) and remained in the pericellular space, allowing oxygen measurements for several hours with a single injection. The oxygen pressure distributions (histograms) were compared with those for Oxyphor G2 in the intravascular (blood plasma) space. In normal muscle, in the lower oxygen pressure region of the histograms (capillary bed) the oxygen pressure difference was small. At higher oxygen pressures in the histograms there were differences consistent with the presence of high flow vessels with oxygen pressures substantially above those of the surrounding interstitial space. In tumors, the oxygen pressures in the two spaces were similar but with large differences among tumors. In mice, anesthesia with ketamine plus xylazine markedly decreased oxygen pressures in the interstitial and intravascular spaces compared to awake or isoflurane anesthetized mice.
Authors:
David F Wilson; William M F Lee; Sosina Makonnen; Sophia Apreleva; Sergei A Vinogradov
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  614     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-02-22     Completed Date:  2008-04-04     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-62     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Dendrimers / chemistry
Extracellular Fluid / metabolism*
Hindlimb
Metalloporphyrins / chemical synthesis,  chemistry
Mice
Molecular Weight
Muscle, Skeletal / blood supply,  metabolism*
Neoplasms, Experimental / blood supply,  metabolism*
Oxygen / analysis*,  blood,  metabolism*
Pressure
Grant Support
ID/Acronym/Agency:
HL081273/HL/NHLBI NIH HHS; NS-31465/NS/NINDS NIH HHS; R01 HL081273/HL/NHLBI NIH HHS; R01 HL081273-01A1/HL/NHLBI NIH HHS; R01 HL081273-02/HL/NHLBI NIH HHS; R01 NS031465/NS/NINDS NIH HHS; R01 NS031465-13A2/NS/NINDS NIH HHS; R01 NS031465-14/NS/NINDS NIH HHS; R01 NS031465-15/NS/NINDS NIH HHS; U54 CA105008-01/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Dendrimers; 0/Metalloporphyrins; S88TT14065/Oxygen
Comments/Corrections

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