Document Detail

Oxygen plasma treatment of polystyrene and Zeonor: substrates for adhesion of patterned cells.
MedLine Citation:
PMID:  19505169     Owner:  NLM     Status:  MEDLINE    
Plastic substrates made of polystyrene (PS) and Zeonor 1060R were treated with oxygen plasma to introduce polar groups (e.g., carbonyl and carboxylic acid) at the surface that render these materials hydrophilic and promote patterned adhesion of HeLa cells. Resultant surfaces were characterized using contact angle goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) to monitor changes in wettability, nanoscale topography, and chemical composition. Biocompatibility of the plastic surfaces was verified through fluorescence microscopy using three fluorophores, Alexa Fluor 488 conjugated to Annexin V, Hoechst 33258, and propidium iodide, indicating cells that undergo apoptosis and necrosis, respectively. The best cell growth was observed on PS treated at 5 W/sccm, for which the viability of adhering HeLa cells exceeded 90%. Patterning was accomplished using an elastomeric microcapillary system (microCS) made of poly(dimethylsiloxane) (PDMS) that consisted of a set of parallel channels to align cells in linear fashion. Densely populated bands were obtained on substrates of both plastic materials when the culture medium contained >2 x 10(5) cells/mL.
Isabelle Beaulieu; Matthias Geissler; Janine Mauzeroll
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  25     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-09     Completed Date:  2009-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7169-76     Citation Subset:  IM    
Chemistry Department, Universite du Quebec a Montreal, 2101 Jeanne-Mance Street, Montreal, QC, H3C 3P8, Canada.
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MeSH Terms
Cell Adhesion*
Hela Cells
Microscopy, Atomic Force
Polystyrenes / chemistry*
Spectrum Analysis / methods
Reg. No./Substance:
0/Polystyrenes; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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