| Oxygen plasma treatment of polystyrene and Zeonor: substrates for adhesion of patterned cells. | |
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MedLine Citation:
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PMID: 19505169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plastic substrates made of polystyrene (PS) and Zeonor 1060R were treated with oxygen plasma to introduce polar groups (e.g., carbonyl and carboxylic acid) at the surface that render these materials hydrophilic and promote patterned adhesion of HeLa cells. Resultant surfaces were characterized using contact angle goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) to monitor changes in wettability, nanoscale topography, and chemical composition. Biocompatibility of the plastic surfaces was verified through fluorescence microscopy using three fluorophores, Alexa Fluor 488 conjugated to Annexin V, Hoechst 33258, and propidium iodide, indicating cells that undergo apoptosis and necrosis, respectively. The best cell growth was observed on PS treated at 5 W/sccm, for which the viability of adhering HeLa cells exceeded 90%. Patterning was accomplished using an elastomeric microcapillary system (microCS) made of poly(dimethylsiloxane) (PDMS) that consisted of a set of parallel channels to align cells in linear fashion. Densely populated bands were obtained on substrates of both plastic materials when the culture medium contained >2 x 10(5) cells/mL. |
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Authors:
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Isabelle Beaulieu; Matthias Geissler; Janine Mauzeroll |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 25 ISSN: 0743-7463 ISO Abbreviation: Langmuir Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-09 Completed Date: 2009-08-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 7169-76 Citation Subset: IM |
Affiliation:
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Chemistry Department, Universite du Quebec a Montreal, 2101 Jeanne-Mance Street, Montreal, QC, H3C 3P8, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion* Hela Cells Humans Microscopy, Atomic Force Oxygen* Polystyrenes / chemistry* Spectrum Analysis / methods X-Rays |
| Chemical | |
Reg. No./Substance:
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0/Polystyrenes; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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