Document Detail


Oxygen modulates the growth of skin fibroblasts.
MedLine Citation:
PMID:  12418928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Elevated oxygen tensions are inhibitory to the growth of skin fibroblasts. Skin fibroblasts grow better at oxygen tensions below 137 mm Hg regardless of seeding density. A wide range of oxygen tensions, including those in the physiological range, strongly modulate the growth of human skin fibroblasts. There were no significant differences between the responses of fetal and postnatal cell lines to changes in ambient oxygen tension. In all cases, higher oxygen tensions significantly impeded cell growth. Seeding cells at 10(4) cells/cm(2) afforded some protection from the deleterious effects of hyperoxia. Oxygen tensions exceeding the amount present in ambient room air also impeded cell growth at this higher seeding density, but the effect did not become significant until the oxygen partial pressure reached 241 mm Hg. At lower oxygen tensions, cells seeded at 10(3) cells/cm(2) grew more rapidly than did cells seeded at 10(4) cells/cm(2). These findings may have implications for the treatment of poorly healing wounds with hyperbaric oxygen.
Authors:
Arthur K Balin; Loretta Pratt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  38     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-11-06     Completed Date:  2003-07-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  305-10     Citation Subset:  IM    
Affiliation:
Laboratory for Investigative Dermatology, The Rockefeller University, New York, New York 10021, USA. akbalin@aol.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Cell Division*
Cell Line
Culture Media
Female
Fetus
Fibroblasts / cytology*,  metabolism
Gestational Age
Humans
Infant
Infant, Newborn
Male
Middle Aged
Oxygen / metabolism*
Pregnancy
Skin / cytology*,  metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
AG00282/AG/NIA NIH HHS; AG04993/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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