| Oxygen metabolites from lavage and interstitial lung cells after inhalation of endotoxin in guinea pigs. | |
| | |
MedLine Citation:
|
PMID: 7950404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Airborne endotoxins play a role in a variety of occupational diseases such as byssinosis and humidifier's disease and are associated with pulmonary and systemic symptoms. An excess generation of oxygen free radicals (including superoxide anion, O2-) by inflammatory cells has been suggested in endotoxemia. We have studied the release of superoxide from guinea-pig lung lavage and interstitial cells and blood monocytes (BMs) at different times after an acute inhalation exposure to bacterial endotoxin. O2- generation was measured by the O2- dismutase-inhibitable reduction of ferricytochrome c, after stimulation with phorbol-myristate-acetate (PMA) or opsonized zymosan (OZ). After endotoxin exposure, the spontaneous release of O2- remained unchanged for the three cell types. From 4 h after exposure until 48 h afterwards, lung lavage cells produced more O2- after PMA or OZ stimulation than did cells from unexposed guinea pigs. The pattern of O2- generation by interstitial cells followed that of lung lavage macrophages. O2- production remained unchanged in BMs at all times. These results suggest that endotoxin inhalation induced a priming of lavage and interstitial cells, mainly macrophages, associated with an increase in O2- production. As O2- production by BMs remained unchanged, this cell is unlikely to be responsible for the systemic symptoms seen after endotoxin inhalation. |
| | |
Authors:
|
V Hsieh; B Amoruso-Marchat; R Rylander; B S Polla |
Related Documents
:
|
19718054 - Reactivating hif prolyl hydroxylases under hypoxia results in metabolic catastrophe and... 3165394 - Peripheral pmn cells in juvenile periodontitis. increased release of elastase and of ox... 20228054 - Protection of cells in physiological oxygen tensions against dna damage-induced apoptosis. 9576744 - Protection from reoxygenation injury by inhibition of rac1. 3913634 - Systemic lymphadenopathy (las) in intravenous drug abusers. histology, immunohistochemi... 14523124 - Investigation of the role of a beta(1-4) agarase produced by pseudoalteromonas gracilis... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International archives of allergy and immunology Volume: 104 ISSN: 1018-2438 ISO Abbreviation: Int. Arch. Allergy Immunol. Publication Date: 1994 May |
Date Detail:
|
Created Date: 1994-11-28 Completed Date: 1994-11-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9211652 Medline TA: Int Arch Allergy Immunol Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 42-7 Citation Subset: IM |
Affiliation:
|
Institute of Social and Preventive Medicine, University Hospital, Geneva, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Administration, Inhalation Animals Bronchoalveolar Lavage Fluid / cytology Cell Count / drug effects Endotoxins / toxicity* Guinea Pigs Lung / cytology*, drug effects*, metabolism Macrophages, Alveolar / drug effects, metabolism Monocytes / drug effects Reactive Oxygen Species / metabolism* Superoxides / metabolism Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Endotoxins; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 16561-29-8/Tetradecanoylphorbol Acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Induction of inflammatory mediator release (12-hydroxyeicosatetraenoic acid) from human platelets by...
Next Document: Immunophenotype of lymphocytes in pericardial fluid from patients with different forms of heart dise...