| Oxygen mass transfer in a human tissue-engineered trachea. | |
| | |
MedLine Citation:
|
PMID: 20378162 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
On June 2008, the first human tissue-engineered trachea replacement was performed using decellularized (de-antigenised) cadaveric donor trachea, seeded with recipient epithelial cells on the internal surface of the graft and mesenchymal stem-cell-derived chondrocytes on the external. During the follow-up, cytological analysis at 4 postoperative days showed a migration of the stem-cells derived chondrocytes from the outer to the inner surface of the first 2 cm of the graft length. With the aim to rationalize these clinical findings, and under the hypothesis that cellular migration is driven by the oxygen gradients developing from the external part of the construct (exposed to O(2) deficiency) towards the better oxygenated epithelial region, an accurate computational model of oxygen transport in the trachea engineered construct was developed and solved using finite element method (FEM). Results confirm that critical limitation to oxygen transport prevalently occurs from proximal to middle section, within the first 2.8 cm of longitudinal length, in good agreement with experimental observation. In the proximal section, recognized as the most critical part of the engineered construct, the severe O(2) mass transfer limitation causes a drastic reduction of the diffusive flux within a distance of 650 microm. At cell density of 1 x 10(7)cells/cm(3), the 30% c.a of the total section area is under oxygen deficiency (O(2) partial pressure below the critical threshold of 38 mmHg). Along the whole tracheal construct, the Thiele modulus ranges within 2.3 and 3.7 in the external chondrocyte compartment, confirming thus the importance of the mass transfer limitation to oxygen diffusion rate. In general, the efficiency of the O(2) transport reduces considerably in the region close to proximal section. |
| | |
Authors:
|
Efrem Curcio; Paolo Macchiarini; Loredana De Bartolo |
Publication Detail:
|
Type: Journal Article Date: 2010-04-08 |
Journal Detail:
|
Title: Biomaterials Volume: 31 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-04-26 Completed Date: 2010-07-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
|
Languages: eng Pagination: 5131-6 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci cubo 17/C, I-87030 Rende (CS), Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport, Active
/
physiology Computer Simulation Humans Models, Biological* Organ Culture Techniques / methods* Oxygen / metabolism* Tissue Engineering / instrumentation*, methods Trachea / growth & development*, metabolism* |
| Chemical | |
Reg. No./Substance:
|
7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Contraction stress related to composite inorganic content.
Next Document: The effect of 3D hydrogel scaffold modulus on osteoblast differentiation and mineralization revealed...