Document Detail

Oxygen-haemoglobin dissociation curve in hypoxic rats of first or second generation.
MedLine Citation:
PMID:  8485927     Owner:  NLM     Status:  MEDLINE    
1. Albino Wistar rats were raised in a normobaric hypoxic environment (10% O2 in N2). Two generations of hypoxic rats were observed for changes in their haemoglobin-oxygen (Hb-O2) dissociation curves (ODC), 2,3-diphosphoglycerate (2,3-DPG), haemoglobin (Hb), and Hill co-efficients at P50 (n50). The first generation were called (H1) and the second generation (H2). The control group (N) had a normoxic environment. 2. Thirty-five rats (13 N rats, 12 H1 rats and 10 H2 rats) were used. The 2,3-DPG was significantly higher in both hypoxic groups when compared with N rats (2.02 +/- 0.51 mmol/L) but 2,3-DPG of H2 rats was significantly lower than that of H1 rats (H1 = 3.48 +/- 0.58 mmol/L and H2 = 2.76 +/- 0.54 mmol/L). The haemoglobin values were N = 2.00 +/- 0.26, H1 = 2.65 +/- 0.32 and H2 = 2.36 +/- 0.30 mmol/L, respectively. 3. We observed considerable differences in Hb-O2 affinity between the three groups of rats. In standard conditions (pH = 7.400; pCO2 = 40 mmHg at 37 degrees C) the H1 rats showed a significantly decreased Hb-O2 affinity (P50,st = 37.0 +/- 1.3 mmHg) when compared with both H2 and N rats; the H2 rats showed a significantly increased Hb-O2 affinity (P50,st = 31.1 +/- 1.5 mmHg) when compared with controls N (P50,st = 34.7 +/- 2.1 mmHg). There were no significant differences in n50 values: N = 2.88 +/- 0.44; H1 = 2.88 +/- 0.77; and H2 = 2.94 +/- 0.67.(ABSTRACT TRUNCATED AT 400 WORDS)
U Quatrini; A Licciardi; G Morici
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  20     ISSN:  0305-1870     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  1993 Apr 
Date Detail:
Created Date:  1993-06-07     Completed Date:  1993-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
Istituto di Fisiologia Umana, Università degli Studi, Palermo, Italy.
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MeSH Terms
Acid-Base Equilibrium
Anoxia / blood*
Carbon Dioxide / blood
Diphosphoglyceric Acids / blood
Hemoglobins / metabolism*
Oxygen / blood*
Rats, Wistar
Reg. No./Substance:
0/Diphosphoglyceric Acids; 0/Hemoglobins; 124-38-9/Carbon Dioxide; 138-81-8/2,3-Diphosphoglycerate; 7782-44-7/Oxygen

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