| Oxygen gradients in CHO cells: measurement and characterization by electron spin resonance. | |
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MedLine Citation:
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PMID: 2550474 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The concentration of oxygen within cells is important in many physiological and pathological processes, but such oxygen-dependent processes are generally studied as a function of the concentration of extracellular oxygen, due to a lack of suitable methods. Using a newly developed technique based on ESR spectroscopy, we show that respiration stimulation of a cell suspension can result in a significant difference between average intracellular and extracellular concentrations of oxygen. These results indicate that studies of oxygen-dependent phenomena in cells may require measurement of intracellular oxygen concentrations and imply that there are mechanisms in cells that restrict the free diffusion of oxygen. |
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Authors:
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J F Glockner; H M Swartz; M A Pals |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 140 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-10-25 Completed Date: 1989-10-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 505-11 Citation Subset: IM |
Affiliation:
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University of Illinois, Department of Medicine, Urbana 61801. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology Cell Line Cricetinae Cytoplasm / metabolism Electron Spin Resonance Spectroscopy Extracellular Space / metabolism Oxygen / physiology* Oxygen Consumption / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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CA-09067/CA/NCI NIH HHS; GM-34250/GM/NIGMS NIH HHS; RR-01811/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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