| Oxygen enhances lethal effect of high-intensity, ultrashort electrical pulses. | |
| | |
MedLine Citation:
|
PMID: 16342277 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The study explored the effect of ambient oxygen on mammalian cell survival after exposure to 10 ns duration, high voltage electrical pulses (nsEP, 80-90 or 120-130 kV/cm; 200-400 pulses per exposure). Cell samples were equilibrated with pure nitrogen, atmospheric air, or pure oxygen prior to the nsEP treatment and were returned to the incubator (air + 5% CO2) shortly after the exposure. The experiments established that survival of hypoxic Jurkat and U937 cells exceeded that of air-equilibrated controls about twofold (P < .01). Conversely, saturation of the medium with oxygen prior to exposure decreased Jurkat cell survival about 1.5 times, P < .01. Attenuation of the cytotoxic effect under hypoxic conditions resembled a well-known effect of oxygen on cell killing by sparsely ionizing radiations and may be indicative of the similarity of underlying cell damage mechanisms. |
| | |
Authors:
|
Kerfoot Walker; Olga N Pakhomova; Juergen Kolb; Karl S Schoenbach; Bruce E Stuck; Michael R Murphy; Andrei G Pakhomov |
Related Documents
:
|
14622987 - Measurement of oxygen consumption in mouse aortic endothelial cells using a micropartic... 10894757 - Novel fluorescent technology platform for high throughput cytotoxicity and proliferatio... 12384967 - Cellular distribution of oxygen sensor candidates-oxidases, cytochromes, k+-channels--i... 1791237 - Differential oxygen sensitivities in g6pdh activities of cultured keloid and normal ski... 15524167 - Autophagy: many paths to the same end. 9888447 - Ex vivo transduction of corneal epithelial progenitor cells using a retroviral vector. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Bioelectromagnetics Volume: 27 ISSN: 0197-8462 ISO Abbreviation: Bioelectromagnetics Publication Date: 2006 Apr |
Date Detail:
|
Created Date: 2006-03-22 Completed Date: 2006-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8008281 Medline TA: Bioelectromagnetics Country: United States |
Other Details:
|
Languages: eng Pagination: 221-5 Citation Subset: IM |
Copyright Information:
|
Published 2005 Wiley-Liss, Inc. |
Affiliation:
|
US Army Medical Research Detachment, Brooks City-Base, San Antonio, Texas, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects*,
radiation effects* Cell Survival / drug effects*, radiation effects* Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Electromagnetic Fields* Humans Jurkat Cells Oxygen / administration & dosage* Radiation Dosage Radiation Tolerance / drug effects, physiology Radiation-Sensitizing Agents / administration & dosage U937 Cells |
| Chemical | |
Reg. No./Substance:
|
0/Radiation-Sensitizing Agents; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Folding and amyloid-fibril formation for a series of human stefins' chimeras: any correlation?
Next Document: Trichloroethylene and dichloroethylene: a critical review of teratogenicity.