| Oxygen electrode-based single antibody amperometric biosensor for qualitative detection of E. coli and bacteria in water. | |
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MedLine Citation:
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PMID: 18324534 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Design and performance of an amperometric biosensor for E. coli O157:H7 that is based on a common dissolved oxygen probe is discussed. Anti-E. coli O157:H7 antibody was conjugated to horseradish peroxidase and immobilized on a nitrocellulose membrane that was placed over the oxygen probe membrane using a custom-fabricated polyvinyl chloride (PVC) insert. Upon bacterial cell binding, a decrease in enzyme activity resulted in a change in oxygen concentration that was detected with a Clark-type oxygen electrode probe. Validation experiments determined the effect of the outer membrane and insert on the Clarke electrode performance and linearity, and the effects of stirring on sensor response. The mechanism of enzymatic disruption is presumably steric hindrance due to binding of the bacterial cell and conformational change in antibody structure. Sampling various dilutions of heat-sterilized E. coli O157:H7 cells in water, as little as 50 bacterial cells/mL could be detected in approximately 20 minutes of sampling and processing procedures. Bacterial concentrations from 0 to 5000 cells/mL were tested, with 2.52 mg/L +/- 0.37 mg/L equivalents of oxygen produced from as few as 50 cells/mL, versus 6.26 +/- 0.64 mg/L when no cells were present in solution. Overall, the developed amperometric biosensor technology offered an efficient means of detection primarily due to its ease of use, cost-effectiveness, portability, and amenability to incorporation at existing water quality gaging stations. |
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Authors:
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Chandra S Theegala; Danyelle D Small; W Todd Monroe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering Volume: 43 ISSN: 1093-4529 ISO Abbreviation: J Environ Sci Health A Tox Hazard Subst Environ Eng Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-03-07 Completed Date: 2008-08-05 Revised Date: 2009-08-14 |
Medline Journal Info:
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Nlm Unique ID: 9812551 Medline TA: J Environ Sci Health A Tox Hazard Subst Environ Eng Country: United States |
Other Details:
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Languages: eng Pagination: 478-87 Citation Subset: IM |
Affiliation:
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Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, Baton Rouge, Louisiana, USA. theegala@lsu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Bacterial
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immunology Bacteria / immunology, isolation & purification* Biosensing Techniques* Colorimetry Electrochemistry / methods* Electrodes* Escherichia coli O157 / immunology, isolation & purification* Oxygen / chemistry* Water Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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