Document Detail


Oxygen diffusion through natural extracellular matrices: implications for estimating "critical thickness" values in tendon tissue engineering.
MedLine Citation:
PMID:  18377199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (Alloderm), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7 x 10(-6) - 2 x 10(-5) cm2/s, Alloderm: 1.9 - 3.1 x 10(-5) cm2/s, and canine fascia lata: 1.6 - 4 x 10(-5) cm2/s. We used the experimental oxygen diffusivity data for these natural ECMs in a mathematical model of oxygen diffusion through a cell-seeded scaffold to estimate the critical size of cell-seeded scaffold that can be cultured in vitro.
Authors:
Caroline Androjna; Jorge E Gatica; Joanne M Belovich; Kathleen A Derwin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  14     ISSN:  1937-3341     ISO Abbreviation:  -     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-10     Completed Date:  2008-06-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  559-69     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, The Cleveland Clinic, Cleveland, Ohio 44195, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / metabolism
Bioreactors
Collagen / metabolism
Diffusion
Dogs
Extracellular Matrix / metabolism*
Fascia Lata / metabolism,  transplantation
Humans
Intestinal Mucosa / transplantation
Intestine, Small / transplantation
Models, Theoretical
Oxygen / metabolism*
Sus scrofa
Tendons*
Tissue Engineering*
Tissue Scaffolds
Grant Support
ID/Acronym/Agency:
R03-AR049858/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Alloderm; 0/Biocompatible Materials; 7782-44-7/Oxygen; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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