Document Detail


Oxygen-dependent inhibition of neutrophil respiratory burst by nitric oxide.
MedLine Citation:
PMID:  8951422     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effect of nitric oxide (NO) on the respiratory burst of neutrophils was examined under different oxygen tensions. Phorbol myristate acetate (PMA) stimulated oxygen consumption and superoxide (O2) generation in neutrophils by a mechanism which was inhibited reversibly by NO. The inhibitory effect of NO increased significantly with a decrease in oxygen tension in the medium. The inhibitory effect of NO was suppressed in medium containing oxyhemoglobin (HbO2), a NO scavenging agent. In contrast, 3-morpholinosydnonimine (SIN-1), a compound that rapidly generates peroxynitrite (ONOO-) from the released NO and O2-, slightly stimulated the PMA-induced respiratory burst. These results suggested that NO, but not ONOO-, might reversibly inhibit superoxide generation by neutrophils especially at physiologically low oxygen tensions thereby decreasing oxygen toxicity particularly in and around hypoxic tissues.
Authors:
S Iha; K Orita; T Kanno; T Utsumi; E F Sato; M Inoue; K Utsumi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  25     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-02-28     Completed Date:  1997-02-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  489-98     Citation Subset:  IM    
Affiliation:
First Department of Surgery, Okayama University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Cytochrome c Group / metabolism
Free Radical Scavengers / metabolism
Neutrophils / metabolism*
Nitric Oxide / metabolism*
Oxidation-Reduction
Oxyhemoglobins / metabolism
Rats
Respiratory Burst / drug effects*
Superoxides / metabolism
Chemical
Reg. No./Substance:
0/Cytochrome c Group; 0/Free Radical Scavengers; 0/Oxyhemoglobins; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides

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