| Oxygen-dependent erythropoietin production by the isolated perfused rat kidney. | |
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MedLine Citation:
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PMID: 1649989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we have investigated the role of oxygen delivery and of classic second messengers on erythropoietin production by the isolated perfused rat kidney. We found that the rat kidney was capable of de novo synthesis of erythropoietin. The erythropoietin production rate was inversely related to the oxygen pressure in the perfusate and increased from 0.17 to 1.85 U erythropoietin h-1 g kidney-1 when arterial PO2 was lowered from 500 mmHg to 30 mmHg. Addition of forskolin (10 microM) and 8-bromo-cGMP (100 microM) to the perfusate elicited significant effects on the renal vascular resistance, but had no significant effect on erythropoietin production. Hypoxia-induced erythropoietin formation, however, was blocked by calmidazolium (1 microM) and W-7 (10 microM), two structurally different putative calmodulin antagonists. Calmidazolium and W-7 had no effect on other functional parameters of the isolated perfused rat kidney such as flow rate, glomerular filtration rate or sodium reabsorption. Our findings suggest that the oxygen-sensing mechanism that controls renal erythropoietin production is primarily located in the kidney itself. A calcium/calmodulin-dependent cellular reaction could be involved in the signal transduction process. |
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Authors:
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H Scholz; H J Schurek; K U Eckardt; A Kurtz; C Bauer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 418 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-08-27 Completed Date: 1991-08-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: 228-33 Citation Subset: IM |
Affiliation:
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Physiologisches Institut der Universität, Zürich, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calmodulin / antagonists & inhibitors Cyclic GMP / analogs & derivatives, pharmacology Erythropoietin / biosynthesis* Forskolin / pharmacology Imidazoles / pharmacology Kidney / blood supply, drug effects, metabolism* Kinetics Male Oxygen / administration & dosage, pharmacology* Rats Second Messenger Systems Sulfonamides / pharmacology Vascular Resistance / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Calmodulin; 0/Imidazoles; 0/Sulfonamides; 11096-26-7/Erythropoietin; 31356-94-2/8-bromocyclic GMP; 57265-65-3/calmidazolium; 65595-90-6/W 7; 66428-89-5/Forskolin; 7665-99-8/Cyclic GMP; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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