Document Detail


Oxygen-controlled automated neural differentiation of mouse embryonic stem cells.
MedLine Citation:
PMID:  23477397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: the aim of this study was to establish a novel automation platform capable of controlling oxygen tension during both the cell-culture and liquid-handling steps of neural differentiation processes.
MATERIALS & METHODS: We built a bespoke automation platform, which enclosed a liquid-handling platform in a sterile, oxygen-controlled environment. An airtight connection was used to transfer cell culture plates to and from an automated oxygen-controlled incubator.
RESULTS: Our results demonstrate that our system yielded comparable cell numbers, viabilities, metabolism profiles and differentiation efficiencies when compared with traditional manual processes. Interestingly, eliminating exposure to ambient conditions during the liquid-handling stage resulted in significant improvements in the yield of MAP2-positive neural cells, indicating that this level of control can improve differentiation processes.
CONCLUSION: This article describes, for the first time, an automation platform capable of maintaining oxygen tension control during both the cell-culture and liquid-handling stages of a 2D embryonic stem cell differentiation process.
Authors:
Paul Mondragon-Teran; Rui Tostoes; Chris Mason; Gary J Lye; Farlan S Veraitch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Regenerative medicine     Volume:  8     ISSN:  1746-076X     ISO Abbreviation:  Regen Med     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-12     Completed Date:  2013-09-26     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  101278116     Medline TA:  Regen Med     Country:  England    
Other Details:
Languages:  eng     Pagination:  171-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Automation
Cell Differentiation / drug effects*
Cell Line
Cell Proliferation / drug effects
Embryonic Stem Cells / cytology*,  drug effects,  metabolism
Mice
Neurons / cytology*,  drug effects,  metabolism
Oxygen / pharmacology*
Reference Standards
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Tubulin; 0/beta3 tubulin, mouse; S88TT14065/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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