| Oxygen consumption rates and oxygen concentration in molt-4 cells and their mtDNA depleted (rho0) mutants. | |
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MedLine Citation:
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PMID: 12547809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Respiratory deficient cell lines are being increasingly used to elucidate the role of mitochondria and to understand the pathophysiology of mitochondrial genetic disease. We have investigated the oxygen consumption rates and oxygen concentration in wild-type (WT) and mitochondrial DNA (mtDNA) depleted (rho(0)) Molt-4 cells. Wild-type Molt-4 cells have moderate oxygen consumption rates, which were significantly reduced in the rho(0) cells. PCMB (p-chloromercurobenzoate) inhibited the oxygen consumption rates in both WT and rho(0) cells, whereas potassium cyanide decreased the oxygen consumption rates only in WT Molt-4 cells. Menadione sodium bisulfite (MSB) increased the oxygen consumption rates in both cell lines, whereas CCCP (carbonyl cyanide m-chlorophenylhydrazone) stimulated the oxygen consumption rates only in WT Molt-4 cells. Superoxide radical adducts were observed in both WT and rho(0) cells when stimulated with MSB. The formation of this adduct was inhibited by PCMB but not by potassium cyanide. These results suggest that the reactive oxygen species (ROS) induced by MSB were at least in part produced via a mitochondrial independent pathway. An oxygen gradient between the extra- and intracellular compartments was observed in WT Molt-4 cells, which further increased when cells were stimulated by CCCP and MSB. The results are consistent with our earlier findings suggesting that such oxygen gradients may be a general phenomenon found in most or all cell systems under appropriate conditions. |
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Authors:
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Jiangang Shen; Nadeem Khan; Lionel D Lewis; Ray Armand; Oleg Grinberg; Eugene Demidenko; Harold Swartz |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 84 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-01-27 Completed Date: 2003-08-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 1291-8 Citation Subset: IM |
Affiliation:
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EPR Center, Department of Diagnostic Radiology, Dartmouth Medical School, Hanover, New Hampshire 03755, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzoates
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pharmacology Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology Cell Respiration / drug effects, physiology* Electron Spin Resonance Spectroscopy / methods* Extracellular Space / metabolism Humans Intracellular Fluid / metabolism Leukemia / genetics, metabolism Mitochondria / drug effects, genetics, metabolism* Mutagenesis, Site-Directed Mutation Oximetry / methods* Oxygen / analysis, metabolism* Oxygen Consumption Potassium Cyanide / pharmacology Reactive Oxygen Species / metabolism Spin Trapping Succinimides / pharmacology Tumor Cells, Cultured / drug effects, metabolism p-Chloromercuribenzoic Acid / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA 231098/CA/NCI NIH HHS; P41 RR11602/RR/NCRR NIH HHS; R01 GM34250/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Benzoates; 0/Methyl 2-((succinimidooxy)carbonyl)benzoate; 0/Reactive Oxygen Species; 0/Succinimides; 151-50-8/Potassium Cyanide; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 59-85-8/p-Chloromercuribenzoic Acid; 7782-44-7/Oxygen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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