Document Detail


Oxygen consumption in undifferentiated versus differentiated adipogenic mesenchymal precursor cells.
MedLine Citation:
PMID:  15766899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To date, no adequate implant material for the correction of soft tissue defects such as after extensive deep burns, tumor resections or in congenital defects is available. A biohybrid composed of viable adipose precursor cells and an optimised matrix could help towards a solution. Morphologically, preadipocytes resemble fibroblasts and have not yet built a large cytoplasmic lipid droplet as found in differentiated adipocytes. Additionally, preadipocytes are smaller than mature adipocytes allowing a quicker revascularization after transplantation. Furthermore, transplanted preadipocytes can form adipose tissue in vivo whereas the transplantation of mature adipocytes often gives poor results, i.e. oil cysts or shrinkage of the transplant. Since these observations point to differences in metabolic activity between preadipocytes and adipocytes, we investigated the oxygen consumption of preadipocytes stimulated to undergo differentiation, and fibroblasts, by measuring the respiration with a Clark-type oxygen electrode. Preadipocytes had a significantly lower oxygen consumption than mature adipocytes. This advantage in respiration and the better revascularization of undifferentiated adipose tissue cells allow the development of innovative transplants and point to preadipocytes as promising tool to improve transplantations in adipose tissue reconstruction.
Authors:
Dennis von Heimburg; Karsten Hemmrich; Sascha Zachariah; Harald Staiger; Norbert Pallua
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  146     ISSN:  1569-9048     ISO Abbreviation:  Respir Physiol Neurobiol     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-15     Completed Date:  2005-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  107-16     Citation Subset:  IM    
Affiliation:
Department of Plastic Surgery and Hand Surgery--Burn Center, University Hospital of the Aachen University of Technology, Pauwelsstr. 30, 52057 Aachen, Germany. dvHeimburg@praxisklinik-kaiserplatz.de
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Adipocytes / physiology*
Adipose Tissue / cytology*
Adult
Animals
Cell Count / methods
Cell Differentiation / physiology*
Cell Proliferation
Cell Respiration
Cells, Cultured
Humans
Mesenchymal Stem Cells / physiology*
Mice
Oxygen Consumption / physiology*
Serum / metabolism
Statistics, Nonparametric
Time Factors
Tissue Engineering

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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