Document Detail


Oxygen consumption in myoglobin-rich and myoglobin-poor isolated fish cardiomyocytes.
MedLine Citation:
PMID:  9493347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The function of myoglobin at the cellular level was investigated by comparing O2 consumption in isolated myoglobin-rich cardiac myocytes from the sea raven (Hemitripterus americanus) and myoglobin-poor myocytes from the ocean pout (Macrozoarces americanus). O2 consumption by sea raven myocytes, 0.21 +/- 0.04 microM O2/10(6) cells.min-1, was significantly higher than O2 consumption by ocean pout myocytes, 0.10 +/- 0.07 microM O2/10(6) cells.min-1 at high PO2. O2 consumption in sea raven myocytes treated with sodium nitrite was not significantly different than that in untreated myocytes at high PO2, but it was significantly lower than controls at low PO2. O2 consumption of sea raven myocytes treated with the mitochondrial uncoupler CCCP was not significantly different from that of control myocytes at high PO2, but it was significantly greater than untreated controls at low PO2. In ocean pout preparations, O2 consumption by nitrite-treated myocytes was significantly higher than that of untreated myocytes at high PO2, but it was not different from that of controls at low PO2. CCCP-treated ocean pout myocytes had a significantly higher oxygen consumption than that of untreated myocytes at high PO2, but oxygen consumption was not different from that of controls at low PO2. The CCCP-activated O2 consumption at low PO2 was myoglobin-dependent in that CCCP alone resulted in a threefold increase in sea raven cells over controls but had no impact on sea raven cells in the presence of nitrite or ocean pout cells treated with CCCP alone. This study further supports the contention that myoglobin only plays an important role in oxygen metabolism at low extracellular PO2's.
Authors:
N J Legate; J R Bailey; W R Driedzic
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  280     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-03-27     Completed Date:  1998-03-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  269-76     Citation Subset:  IM    
Affiliation:
Department of Biology, Mount Allison University, Sackville, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
Cells, Cultured
Fishes
Mitochondria / metabolism
Myocardium / cytology,  metabolism*
Myoglobin / physiology*
Oxidation-Reduction
Oxygen Consumption / physiology*
Sodium Nitrite / pharmacology
Uncoupling Agents / pharmacology
Chemical
Reg. No./Substance:
0/Myoglobin; 0/Uncoupling Agents; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 7632-00-0/Sodium Nitrite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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