Document Detail


Oxygen consumption in the avascular guinea pig retina.
MedLine Citation:
PMID:  8853355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxygen consumption across the retina of a mammal with a naturally avascular retina has not previously been investigated. The oxygen consumption across the avascular retina of the guinea pig was measured in vivo by spatial analysis of the intraretinal oxygen profile. The avascular nature of the guinea pig retina allows the inner retina to be included in the analysis without disrupting the normal physiological state of the retina. Oxygen-sensitive microelectrodes (1-micron tip) were used to make high-resolution measurements of oxygen tension through the retina of anesthetized, mechanically ventilated guinea pigs (n = 10). Oxygen profiles were then analyzed in terms of oxygen tension as a function of distance from the choriocapillaris/Bruch's membrane, and the data were fitted to an established mathematical model of retinal oxygen consumption. The average oxygen consumption of the full thickness of the guinea pig retina was 1.1 +/- 0.09 ml O2.min-1 x 100 g-1 (n = 10). The average oxygen consumption of the outer half of the retina was 2.07 +/- 0.17 ml O2.min-1 x 100 g-1, while that of the inner half was only 0.12 +/- 0.04 ml O2.min-1 x 100 g-1. A localized region of high oxygen consumption was identified in the outer retina in every case, and this region accounted for an average of 93.9 +/- 2.0% of the total retinal oxygen consumption. Only 5.2 +/- 1.4% of the total oxygen consumption was attributable to the inner half of the retina. When choroidal oxygen tension was increased via a combination of systemic hyperoxia and hypercapnia, high oxygen levels could be sustained in all retinal layers. Under these conditions of an excess oxygen supply, the inner retina still consumed only 0.45 +/- 0.11 ml O2.min-1 x 100 g-1, which was 13.8 +/- 2.5% of the total retinal oxygen consumed. The relatively low oxygen consumption in the inner retina of the guinea pig may reflect an interesting adaptation to the constraints imposed by the absence of a retinal circulation in this species.
Authors:
S Cringle; D Y Yu; V Alder; E N Su; P Yu
Related Documents :
22728605 - Meta-analysis of the effect of exercise programs for individuals with intellectual disa...
3709245 - Evaluation of the pendant oxygen-conserving nasal cannula during exercise.
14961265 - Survival of f2 transgenic common carp (cyprinus carpio) containing prsvrtgh1 complement...
18599385 - Breath-by-breath changes of lung oxygen stores at rest and during exercise in humans.
18840395 - Long term exercise capacity in patients with hypertrophic cardiomyopathy treated with p...
22612425 - Effect of cooling during inter-exercise periods on subsequent intramuscular water movem...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  271     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-12-05     Completed Date:  1996-12-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1162-5     Citation Subset:  IM    
Affiliation:
Lions Eye Institute, Centre for Ophthalmology and Visual Science, University of Western Australia, Nedlands, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Guinea Pigs / metabolism*
Hypercapnia / metabolism
Hyperoxia / metabolism
Oxygen / metabolism
Oxygen Consumption*
Partial Pressure
Retina / metabolism*
Retinal Vessels*
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Contribution of Na+/Ca2+ exchange to action potential of human atrial myocytes.
Next Document:  Nitrite oxidation of myoglobin in perfused myocardium: implications for energy coupling in respirati...