| Oxocarboxylic acids, pyridine nucleotide-linked oxidoreductases and serum factors in regulation of cell proliferation. | |
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MedLine Citation:
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PMID: 575536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When serum is made rate-limiting for clonal multiplication of human diploid fibroblasts, the presence of a 2-oxocarboxylic acid in the medium becomes essential. The requirement is independent of the 20 amino acids and glucose. Glyoxylic, pyruvic, 2-oxoglutaric, and oxalacetic acids are most effective. The types of 2-oxocarboxylic acids that support multiplication are oxidized substrates for several, pyridine nucleotide-linked intracellular oxidoreductases. The requirement is not satisfied by carboxylic acids, oxidized substrates for oxidoreductases that are not lniked to pyridine nucleotides, or by nonspecific electron acceptors. The quantitative requirement for 2-oxocarboxylic acids in cell multiplication is markedly affected by the concentration of serum proteins in the medium. Therefore, 2-oxocarboxylic acid metabolism may be related to the mechanism by which serum growth factors regulate cell multiplication. |
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Authors:
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W L McKeehan; K A McKeehan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular physiology Volume: 101 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1979 Oct |
Date Detail:
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Created Date: 1980-06-16 Completed Date: 1980-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9-16 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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metabolism Animals Blood Proteins / pharmacology* Cattle Cell Division* / drug effects Cell Line Culture Media Fibroblasts / cytology, drug effects, metabolism Glucose / metabolism Humans Keto Acids / metabolism* Lung Oxidoreductases / metabolism* Pyruvates / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Blood Proteins; 0/Culture Media; 0/Keto Acids; 0/Pyruvates; 50-99-7/Glucose; EC 1.-/Oxidoreductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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